Senescent cells in rabbit, nutria and chinchilla testes—Results from histochemical and immunohistochemical studies

• Published in: Animal reproduction science 2021-03, Vol.226, p.106701-106701, Article 106701
• Main Authors: Lustofin, Krzysztof, Niedbala, Piotr, Pawlicki, Piotr, Tuz, Ryszard, Płachno, Bartosz J., Profaska-Szymik, Magdalena, Galuszka, Anna, Stolarczyk, Piotr, Gorowska-Wojtowicz, Ewelina, Kotula-Balak, Malgorzata
• Format: Article
• Language: English
• Subjects: Chinchilla; Nutria; Rabbit; Senescence; Testes
• ISSN: 0378-4320; 1873-2232
• DOI: 10.1016/j.anireprosci.2021.106701

•In testes of mature rabbit, nutria and chinchilla, abnormal seminiferous tubules are present.•Senescence signal is located especially in cells of altered tubules.•Expression of senescence markers is the highest in nutria and chinchilla testes.•No species –specific differences in expression of apoptosis and autophagy markers are found. Rabbit, nutria and chinchilla testes were evaluated to compare testicular cellular senescence. There were no major species-specific differences in structure of either seminiferous tubules or interstitial tissue. There, however, were occasional abnormalities in seminiferous tubule structure with there being multinucleated and exfoliated cells present in rabbit testes. Furthermore, there were seminiferous tubules without a lumen that were filled with premeiotic/meiotic cells in nutria; and tubules with vacuolization with there being no post-meiotic cells in chinchillas. There were no differences in distribution or content of acids, total proteins and polysaccharides in the testis of any of the three species. Results using comparative immunohistochemistry procedures indicated the testes contained a few senescent cells in seminiferous tubules with typical morphology and there was a large number of senescent cells in seminiferous tubules of nutrias and chinchillas that had an abnormal structure (P