Development and Genetic Engineering of Hyper-Producing Microbial Strains for Improved Synthesis of Biosurfactants

• Published in: Molecular biotechnology 2021-04, Vol.63 (4), p.267-288
• Main Authors: Jimoh, Abdullahi Adekilekun, Senbadejo, Tosin Yetunde, Adeleke, Rasheed, Lin, Johnson
• Format: Article
• Language: English
• Subjects: Biochemistry; Biological Techniques; Biosurfactants; Biotechnology; Cell Biology; Chemistry; Chemistry and Materials Science; Gene expression; Genes; Genetic engineering; Genetics; Global economy; Host systems; Human Genetics; Microbiology; Microorganisms; Molecular biology; Molecular dynamics; Protein Science; Recombinants; Review; Strains (organisms); Surfactants; Synthesis
• ISSN: 1073-6085; 1559-0305
• DOI: 10.1007/s12033-021-00302-1

Current research energies are fixated on the synthesis of environmentally friendly and non-hazardous products, which include finding and recognizing biosurfactants that can substitute synthetic surfactants. Microbial biosurfactants are surface-active compounds synthesized intracellularly or extracellularly. To use biosurfactants in various industries, it is essential to understand scientific engagements that demonstrate its potentials as real advancement in the 21st century. Other than applying a substantial effect on the world economic market, engineered hyper-producing microbial strains in combination with optimized cultivation parameters have made it probable for many industrial companies to receive the profits of 'green' biosurfactant innovation. There needs to be an emphasis on the worldwide state of biosurfactant synthesis, expression of biosurfactant genes in expressive host systems, the recent developments, and prospects in this line of research. Thus, molecular dynamics with respect to genetic engineering of biosynthetic genes are proposed as new biotechnological tools for development, improved synthesis, and applications of biosurfactants. For example, mutant and hyper-producing recombinants have been designed efficaciously to advance the nature, quantity, and quality of biosurfactants. The fastidious and deliberate investigation will prompt a comprehension of the molecular dynamics and phenomena in new microorganisms. Throughout the decade, valuable data on the molecular genetics of biosurfactant have been produced, and this solid foundation would encourage application-oriented yields of the biosurfactant production industry and expand its utilization in diverse fields. Therefore, the conversations among different interdisciplinary experts from various scientific interests such as microbiology, biochemistry, molecular biology, and genetics are indispensable and significant to accomplish these objectives.