Bio-nanocomposite based highly sensitive and label-free electrochemical immunosensor for endometriosis diagnostics application

• Published in: Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2021-06, Vol.139, p.107740-107740, Article 107740
• Main Authors: Kalyani, Thangapandi, Sangili, Arumugam, Nanda, Amalesh, Prakash, Sengodu, Kaushik, Ajeet, Kumar Jana, Saikat
• Format: Article
• Language: English
• Subjects: Antibodies; Antigens; Bio-nanocomposite; Biomarkers; CA19-9 biomarker; Carbohydrates; Chitosan; Crosslinking; Diagnostics composite; Electrochemical biosensor; Electrochemistry; Electrodes; Endometriosis; Enzyme-linked immunosorbent assay; Glassy carbon; Immune-sensing; Immunosensors; Iron oxides; Magnetite; Multi wall carbon nanotubes; Nano-sensors; Nanocomposites; Nanoparticles; Optimization; Sorbents
• ISSN: 1567-5394; 1878-562X
• DOI: 10.1016/j.bioelechem.2021.107740

[Display omitted] •A bio-nanocomposite platform is explored to fabricates a highly sensitive electrochemical immunosensor,•For the first time, this nano-enabled sensor detects CA 19–9, a biomarker for endometriosis diagnostics,•Presented CA19-9 immunosensor is affordable and exhibited a detection limit as 0.163 pg/mL.•Sensing performance of CA19-9 immunosensor is tested using real sample and validated using ELISA,•Outcomes confirmed suitability of this sensor for clinical applications to diagnose endometriosis. In this research, for the first time, a bio-nanocomposites based highly sensitive and label-free electrochemical immunosensor is reported with the aim of endometriosis diagnostics application. Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19–9 (CA19-9), a potential biomarker for endometriosis diagnostics. Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19–9 and exhibited a high sensitivity as (2.55 µA pg−1 cm−1), a detection limit of 0.163 pg mL−1, detection range from 1.0 pg mL−1 to 100 ng mL−1. Our fabricated electrochemical AbsCA19-9/CS-MWCNT-Fe3O4 immunosensor performed CA19-9 sensing in physiological range and at a very level which suggest it application for early-stage diagnostics, diseases monitoring, and optimization of therapy. To claim the clinical application, our sensor was tested using real samples and sensing performance was validated using enzyme-linked immune-sorbent assay (ELISA). The results of the studies projected AbsCA19-9/CS-MWCNT-Fe3O4 electrochemical CA19-9 immunosensor as a potential and affordable alternate of conventional techniques like ELISA. We believe that our fabricated sensor can be the plane of a disease’s management program due to affordable, rapid, label-free, and sensitive detection of a targeted biomarker.