Immunohistochemical expression of HER ‐ 2 and Ki ‐ 67 in granulosa cell tumor in bitches

Granulosa cell tumour, an ovarian neoplasm of stromal origin, is an important tumour related to oestrogenic dominance syndrome and cystic endometrial hyperplasia–pyometra complex. In order to analyse ovarian tumour´s malignant potential, immunohistochemical markers can be used, such as anti‐HER2 and...

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Bibliographic Details
Published in:Reproduction in domestic animals 2021-04, Vol.56 (4), p.667-672
Main Authors: Matos, Ana Carina Heil da Silva, Consalter, Angélica, Santos Batista, Bárbara Paula, Fonseca, Ana Beatriz Monteiro, Ferreira, Ana Maria Reis, Leite, Juliana da Silva
Format: Article
Language:English
Subjects:
Antibodies
anti‐HER‐2
anti‐Ki‐67
canine
Endometrium
ErbB-2 protein
granulosa cell
Hyperplasia
Labeling
Markers
Ovaries
ovary
Pleomorphism
Tumors
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Summary:Granulosa cell tumour, an ovarian neoplasm of stromal origin, is an important tumour related to oestrogenic dominance syndrome and cystic endometrial hyperplasia–pyometra complex. In order to analyse ovarian tumour´s malignant potential, immunohistochemical markers can be used, such as anti‐HER2 and anti‐Ki‐67. The aim of this study was to evaluate the expression of immunohistochemical markers HER‐2 and Ki‐67 in granulosa cell tumour from bitches´ ovaries. In HER‐2 immunomarker analysis using the HercepTest® method, most tumours were classified as 2+ (moderate labelling). Concerning Ki‐67 immunomarker, only one case was described as having a high proliferative index. An association was found between immunostained cell percentage by anti‐HER‐2 antibodies and high pleomorphism, represented by the pattern of follicular/trabecular tumour arrangement. There was no correlation between anti‐Ki‐67 and anti‐HER‐2 antibody immunostaining intensities, probably due to only one case with a high Ki‐67 index. With an effective protocol for HER‐2 and Ki‐67 immunohistochemical identification in granulosa cell tumours in bitches, it was possible to characterize this neoplasm proliferation profile.
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.13903