Gene expression profiles of pro-inflammatory mediators in the conjunctiva of patients with epiblepharon

Purpose To investigate the gene expression of pro-inflammatory mediators in the conjunctiva of pediatric patients with epiblepharon in a case-control study. Methods Twenty healthy controls and 15 pediatric patients with epiblepharon were enrolled from April 23, 2020 to June 15, 2020. Epiblepharon se...

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Bibliographic Details
Published in:Graefe's archive for clinical and experimental ophthalmology 2021-07, Vol.259 (7), p.2027-2033
Main Authors: Kim, Bo Ram, Seo, Yuri, Lee, Hyun Jung, Chung, So-Hyang, Yoon, Jin Sook
Format: Article
Language:English
Subjects:
Conjunctiva
Cornea
Gelatinase B
Gene expression
IL-1β
Inflammation
Interleukin 6
Matrix metalloproteinase
Medicine
Medicine & Public Health
Metalloproteinase
Mucin
Oculoplastics and Orbit
Ophthalmology
Patients
Pediatrics
Polymerase chain reaction
Reverse transcription
Surgery
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Summary:Purpose To investigate the gene expression of pro-inflammatory mediators in the conjunctiva of pediatric patients with epiblepharon in a case-control study. Methods Twenty healthy controls and 15 pediatric patients with epiblepharon were enrolled from April 23, 2020 to June 15, 2020. Epiblepharon severity was divided into class I–III (least to moderate severity) and class IV (most severe). We obtained impression cytologic specimens from the medial palpebral conjunctiva of the participants to measure the gene expression of interleukin ( IL )-1β, IL-6 , matrix metalloproteinase 9 ( MMP9 ), and mucin 5AC ( MUC5AC ) using quantitative reverse transcription polymerase chain reaction. Results The mean age in the epiblepharon group was 9 years (range 7.5–11 years), and that in the healthy control group was 9.5 years (range 8–11.3 years). IL-1β , IL-6 , and MMP9 expression levels were 2.08 ( p  
ISSN:0721-832X
1435-702X
DOI:10.1007/s00417-021-05089-0