Silver-doped bioactive glass particles for in vivo bone tissue regeneration and enhanced methicillin-resistant Staphylococcus aureus (MRSA) inhibition

• Published in: Materials Science & Engineering C 2021-01, Vol.120, p.111693-111693, Article 111693
• Main Authors: Pajares-Chamorro, Natalia, Wagley, Yadav, Maduka, Chima V., Youngstrom, Daniel W., Yeger, Alyssa, Badylak, Stephen F., Hammer, Neal D., Hankenson, Kurt, Chatzistavrou, Xanthippi
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Antibiotics; Bacteria; Bacterial diseases; Bacterial infections; Bioglass; Biological activity; Biological properties; Biomaterials; Biomedical materials; Bone biomaterials; Bone growth; Bone healing; Bone marrow; Bone Regeneration; Calvarial bone growth; Cell differentiation; Cell growth; Cell proliferation; Cell viability; Cytotoxicity; Differentiation (biology); Drug resistance; Glass; Health risks; In vivo methods and tests; Infections; Materials science; Methicillin; Methicillin-Resistant Staphylococcus aureus; Mice; Microparticles; Osteogenesis; Regeneration; Regeneration (physiology); Risk analysis; Risk factors; Silver; Silver - pharmacology; Silver doped bioactive glass; Sol-gel processes; Staphylococcus aureus; Staphylococcus infections; Stromal cells; Synergistic antibacterial properties; Tissue engineering
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2020.111693

Infection is a significant risk factor for failed healing of bone and other tissues. We have developed a sol-gel (solution-gelation) derived bioactive glass doped with silver ions (Ag-BG), tailored to provide non-cytotoxic antibacterial activity while significantly enhancing osteoblast-lineage cell growth in vitro and bone regeneration in vivo. Our objective was to engineer a biomaterial that combats bacterial infection while maintaining the capability to promote bone growth. We observed that Ag-BG inhibits bacterial growth and potentiates the efficacy of conventional antibiotic treatment. Ag-BG microparticles enhance cell proliferation and osteogenic differentiation in human bone marrow stromal cells (hBMSC) in vitro. Moreover, in vivo tests using a calvarial defect model in mice demonstrated that Ag-BG microparticles induce bone regeneration. This novel system with dual biological and advanced antibacterial properties is a promising therapeutic for combating resistant bacteria while triggering new bone formation. [Display omitted] •Ag-BG is antibacterial against MRSA in a biofilm-simulated environment.•Ag-BG synergizes with vancomycin, inhibiting significantly planktonic MRSA at very low concentrations of the two agents.•The critical concentrations were identified for optimum combinatorial therapy.•Ag-BG enhances cell proliferation and differentiation.•Ag-BG induces bone regeneration in in vivo mouse calvarial model.