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Immobilization of Serratia plymuthica by ionic gelation and cross-linking with transglutaminase for the conversion of sucrose into isomaltulose
Isomaltulose is an alternative sugar obtained from sucrose using some bacteria producing glycosyltransferase. This work aimed to optimize conditions for the immobilization of Serratia plymuthica through ionic gelation and cross-linking by transglutaminase using the sequential experimental strategy f...
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| Published in: | Bioprocess and biosystems engineering 2021-06, Vol.44 (6), p.1109-1118 |
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| Main Authors: | , , , |
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| cited_by | cdi_FETCH-LOGICAL-c412t-3e542bb7df73b74c1c42fb3d4fe084c50224a4854fe0a04ea99c09eff98f148d3 |
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| cites | cdi_FETCH-LOGICAL-c412t-3e542bb7df73b74c1c42fb3d4fe084c50224a4854fe0a04ea99c09eff98f148d3 |
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| container_issue | 6 |
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| container_title | Bioprocess and biosystems engineering |
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| creator | Carvalho, Priscila Hoffmann Kawaguti, Haroldo Yukio de Souza, Weysser Felipe Cândido Sato, Hélia Harumi |
| description | Isomaltulose is an alternative sugar obtained from sucrose using some bacteria producing glycosyltransferase. This work aimed to optimize conditions for the immobilization of
Serratia plymuthica
through ionic gelation and cross-linking by transglutaminase using the sequential experimental strategy for the conversion of sucrose into isomaltulose. The effect of five variables (concentrations of cell mass, alginate, gelatin, transglutaminase, and calcium chloride) was studied, as well as the interactions between them on the matrix composition for the
S. plymuthica
immobilization. Three experimental designs were used to optimize the concentrations of each variable to obtain higher concentration of isomaltulose. A high conversion of sucrose into isomaltulose (71.04%) was obtained by the cells immobilized in a matrix composed of alginate (1.7%), CaCl
2
(0.25 mol/L), gelatin (0.5%), transglutaminase (3.5%) and cell mass (33.5%). As a result, the transglutaminase application as a cross-linking agent improved the immobilization of
Serratia plymuthica
cells and the conversion of sucrose into isomaltulose. |
| doi_str_mv | 10.1007/s00449-021-02513-x |
| format | article |
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Serratia plymuthica
through ionic gelation and cross-linking by transglutaminase using the sequential experimental strategy for the conversion of sucrose into isomaltulose. The effect of five variables (concentrations of cell mass, alginate, gelatin, transglutaminase, and calcium chloride) was studied, as well as the interactions between them on the matrix composition for the
S. plymuthica
immobilization. Three experimental designs were used to optimize the concentrations of each variable to obtain higher concentration of isomaltulose. A high conversion of sucrose into isomaltulose (71.04%) was obtained by the cells immobilized in a matrix composed of alginate (1.7%), CaCl
2
(0.25 mol/L), gelatin (0.5%), transglutaminase (3.5%) and cell mass (33.5%). As a result, the transglutaminase application as a cross-linking agent improved the immobilization of
Serratia plymuthica
cells and the conversion of sucrose into isomaltulose.</description><identifier>ISSN: 1615-7591</identifier><identifier>EISSN: 1615-7605</identifier><identifier>DOI: 10.1007/s00449-021-02513-x</identifier><identifier>PMID: 33547961</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Alginates ; Alginic acid ; Bacteria ; Biotechnology ; Calcium alginate ; Calcium chloride ; Chemistry ; Chemistry and Materials Science ; Conversion ; Crosslinking ; Environmental Engineering/Biotechnology ; Food Science ; Gelatin ; Gelation ; Glycosyltransferase ; Immobilization ; Industrial and Production Engineering ; Industrial Chemistry/Chemical Engineering ; Research Paper ; Serratia plymuthica ; Sucrose</subject><ispartof>Bioprocess and biosystems engineering, 2021-06, Vol.44 (6), p.1109-1118</ispartof><rights>This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2021</rights><rights>This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-3e542bb7df73b74c1c42fb3d4fe084c50224a4854fe0a04ea99c09eff98f148d3</citedby><cites>FETCH-LOGICAL-c412t-3e542bb7df73b74c1c42fb3d4fe084c50224a4854fe0a04ea99c09eff98f148d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33547961$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carvalho, Priscila Hoffmann</creatorcontrib><creatorcontrib>Kawaguti, Haroldo Yukio</creatorcontrib><creatorcontrib>de Souza, Weysser Felipe Cândido</creatorcontrib><creatorcontrib>Sato, Hélia Harumi</creatorcontrib><title>Immobilization of Serratia plymuthica by ionic gelation and cross-linking with transglutaminase for the conversion of sucrose into isomaltulose</title><title>Bioprocess and biosystems engineering</title><addtitle>Bioprocess Biosyst Eng</addtitle><addtitle>Bioprocess Biosyst Eng</addtitle><description>Isomaltulose is an alternative sugar obtained from sucrose using some bacteria producing glycosyltransferase. This work aimed to optimize conditions for the immobilization of
Serratia plymuthica
through ionic gelation and cross-linking by transglutaminase using the sequential experimental strategy for the conversion of sucrose into isomaltulose. The effect of five variables (concentrations of cell mass, alginate, gelatin, transglutaminase, and calcium chloride) was studied, as well as the interactions between them on the matrix composition for the
S. plymuthica
immobilization. Three experimental designs were used to optimize the concentrations of each variable to obtain higher concentration of isomaltulose. A high conversion of sucrose into isomaltulose (71.04%) was obtained by the cells immobilized in a matrix composed of alginate (1.7%), CaCl
2
(0.25 mol/L), gelatin (0.5%), transglutaminase (3.5%) and cell mass (33.5%). As a result, the transglutaminase application as a cross-linking agent improved the immobilization of
Serratia plymuthica
cells and the conversion of sucrose into isomaltulose.</description><subject>Alginates</subject><subject>Alginic acid</subject><subject>Bacteria</subject><subject>Biotechnology</subject><subject>Calcium alginate</subject><subject>Calcium chloride</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Conversion</subject><subject>Crosslinking</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Food Science</subject><subject>Gelatin</subject><subject>Gelation</subject><subject>Glycosyltransferase</subject><subject>Immobilization</subject><subject>Industrial and Production Engineering</subject><subject>Industrial Chemistry/Chemical Engineering</subject><subject>Research Paper</subject><subject>Serratia plymuthica</subject><subject>Sucrose</subject><issn>1615-7591</issn><issn>1615-7605</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kc1u1DAUhS1ERUvhBVggS2zYhPp3kixRBaVSJRbA2nKc6xkXxx5sp3T6En3lepopSCxYWPb1_c65lg9Cbyj5QAlpzzIhQvQNYbQuSXlz-wyd0BWVTbsi8vnTWfb0GL3M-ZoQKjtGXqBjzqVo-xU9QfeX0xQH592dLi4GHC3-BinVQuOt301z2Tij8bDDtesMXoNfQB1GbFLMufEu_HRhjX-7ssEl6ZDXfi56ckFnwDYmXDaATQw3kPJhRp73WsAulIhdjpP2Zfb15hU6stpneH3YT9GPz5--n39prr5eXJ5_vGqMoKw0HKRgw9COtuVDKww1gtmBj8IC6YSRhDGhRSf3tSYCdN8b0oO1fWep6EZ-it4vvtsUf82Qi5pcNuC9DhDnrJjoWiol72VF3_2DXsc5hfo6xSSngjHOaKXYQj1-SgKrtslNOu0UJWofl1riUjUu9RiXuq2itwfreZhg_CN5yqcCfAFybYU1pL-z_2P7ABZWpGY</recordid><startdate>20210601</startdate><enddate>20210601</enddate><creator>Carvalho, Priscila Hoffmann</creator><creator>Kawaguti, Haroldo Yukio</creator><creator>de Souza, Weysser Felipe Cândido</creator><creator>Sato, Hélia Harumi</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20210601</creationdate><title>Immobilization of Serratia plymuthica by ionic gelation and cross-linking with transglutaminase for the conversion of sucrose into isomaltulose</title><author>Carvalho, Priscila Hoffmann ; 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This work aimed to optimize conditions for the immobilization of
Serratia plymuthica
through ionic gelation and cross-linking by transglutaminase using the sequential experimental strategy for the conversion of sucrose into isomaltulose. The effect of five variables (concentrations of cell mass, alginate, gelatin, transglutaminase, and calcium chloride) was studied, as well as the interactions between them on the matrix composition for the
S. plymuthica
immobilization. Three experimental designs were used to optimize the concentrations of each variable to obtain higher concentration of isomaltulose. A high conversion of sucrose into isomaltulose (71.04%) was obtained by the cells immobilized in a matrix composed of alginate (1.7%), CaCl
2
(0.25 mol/L), gelatin (0.5%), transglutaminase (3.5%) and cell mass (33.5%). As a result, the transglutaminase application as a cross-linking agent improved the immobilization of
Serratia plymuthica
cells and the conversion of sucrose into isomaltulose.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>33547961</pmid><doi>10.1007/s00449-021-02513-x</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 1615-7591 |
| ispartof | Bioprocess and biosystems engineering, 2021-06, Vol.44 (6), p.1109-1118 |
| issn | 1615-7591 1615-7605 |
| language | eng |
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| source | Springer Nature |
| subjects | Alginates Alginic acid Bacteria Biotechnology Calcium alginate Calcium chloride Chemistry Chemistry and Materials Science Conversion Crosslinking Environmental Engineering/Biotechnology Food Science Gelatin Gelation Glycosyltransferase Immobilization Industrial and Production Engineering Industrial Chemistry/Chemical Engineering Research Paper Serratia plymuthica Sucrose |
| title | Immobilization of Serratia plymuthica by ionic gelation and cross-linking with transglutaminase for the conversion of sucrose into isomaltulose |
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