Hemostatic performance and biocompatibility of chitosan-based agents in experimental parenchymal bleeding

• Published in: Materials Science & Engineering C 2021-01, Vol.120, p.111740-111740, Article 111740
• Main Authors: Deineka, V., Sulaieva, O., Pernakov, N., Radwan-Pragłowska, J., Janus, L., Korniienko, V., Husak, Ye, Yanovska, A., Liubchak, I., Yusupova, A., Piątkowski, M., Zlatska, A., Pogorielov, M.
• Format: Article
• Language: English
• Subjects: Aerogel; Aerogels; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Biocompatibility; Biodegradation; Bleeding; Chitosan; Chitosan - pharmacology; Complications; Copolymers; Electrospinning; Hemorrhage - drug therapy; Hemostasis; Hemostatics - pharmacology; Humans; Inflammation; Materials science; Natural polymers; Performance degradation; Polymers; Surgery
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2020.111740

The uncontrolled parenchymatic bleeding is still a cause of serious complications in surgery and require new effective hemostatic materials. In recent years, numerous chitosan-based materials have been intensively studied for parenchymatic bleeding control but still require to increased safety and effectiveness. The current research is devoted to new hemostatic materials made of natural polymer (chitosan) developed using electrospinning and microwave-assisted methods. Hemostatic performance, biocompatibility, degradation, and in-vivo effectiveness were studied to assess functional properties of new materials. Chitosan-based agents demonstrated considerable hemostatic performance, moderate biodegradation pace and high biocompatibility in vitro. Using the electrospinning-made chitosan-copolymer significantly improved in vivo biocompatibility and degradation of Chitosan-based agents that provides opportunities for its implementation for visceral bleeding management. Chitosan aerogel could be effectively applied in hemostatic patch development due to high antibacterial activity but it is not recommended for visceral application due to moderate inflammatory effect and slow degradation. [Display omitted] •Chitosan aerogel and electrospun membrane have considerable hemostatic performance.•Chitosan electrospun membrane demonstrate high pore area and advanced interaction with whole blood.•Chitosan aerogel was associated with moderate inflammatory reaction and revealed slow degradation.•Electrospinning-made Ch-copolymer demonstrated high in vivo biocompatibility and degradation pace.