Purification, characterization, and in vitro antitumor activity of a novel glucan from the purple sweet potato Ipomoea Batatas (L.) Lam

• Published in: Carbohydrate polymers 2021-04, Vol.257, p.117605-117605, Article 117605
• Main Authors: Ji, Chenfeng, Zhang, Ziyi, Zhang, Baihui, Chen, Jinrui, Liu, Rongyu, Song, Dongxue, Li, Wenlan, Lin, Na, Zou, Xiang, Wang, Jin, Guo, Shoudong
• Format: Article
• Language: English
• Subjects: Atomic force microscopy; Circular dichroism; Microstructure; NMR spectrometry; Purple sweet potato; Scanning electron microscope
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2020.117605

•A glucan (18.3 kDa) from purple sweet potato is composed of →4)Glcp(1→ glycosyls.•It branches at O-2, O-3, and O-6 positions with α-d-Glcp(1→ residue as side chain.•Its particle size is concentrated at 230 nm and has no triple-helix conformation.•Its height and width rangs from 1.0 to 6.1 nm and 65 to 210 nm, respectively.•Its maximum ring diameter and chain length is ∼800 nm and ∼7.0 μm, respectively. A novel glucan PSPP-1 (18.3 kDa) was purified from the foot tuber of purple sweet potato Ipomoea Batatas (L.) Lam. Its backbone was composed of →4)-α-d-Glcp(1→ glycosyl, and branching at the O-2, O-3, and O-6 positions with α-d-Glcp(1→ residues. X-ray diffraction experiment showed that PSPP-1 existed as an amorphous form. Its microstructure was detected via scanning electron microscopy. Its particle size was mainly concentrated at 230 nm in water. Congo red and circular dichroism experiments showed there was no triple-helix conformation. Atomic force microscopy data suggested that its height and width ranged from 1.0 to 6.1 nm and 65 to 210 nm, respectively; its maximum ring diameter and chain length was ∼800 nm and ∼7.0 μm, respectively. Furthermore, it exhibited inhibitory activities on HepG2, LOVO, and MCF-7 cells. Collectively, our data are useful for understanding the structural characteristics of sweet potato polysaccharides, and their application in foods and pharmaceutical areas.