cDNA TRAP display for rapid and stable in vitro selection of antibody-like proteins

We developed a cDNA TRAP display for the rapid selection of antibody-like proteins in various conditions. By modifying the original puromycin linker in the TRAP display, a monobody was covalently attached to the cDNA. As a proof-of-concept, we demonstrated a rapid model selection of an anti-EGFR1 mo...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2021-03, Vol.57 (19), p.2416-2419
Main Authors: Kondo, Taishi, Eguchi, Minori, Kito, Seita, Fujino, Tomoshige, Hayashi, Gosuke, Murakami, Hiroshi
Format: Article
Language:English
Subjects:
Antibodies
Proteins
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Summary:We developed a cDNA TRAP display for the rapid selection of antibody-like proteins in various conditions. By modifying the original puromycin linker in the TRAP display, a monobody was covalently attached to the cDNA. As a proof-of-concept, we demonstrated a rapid model selection of an anti-EGFR1 monobody in a solution containing ribonuclease.
ISSN:1359-7345
1364-548X
DOI:10.1039/d0cc07541h