Fbf1 regulates mouse oocyte meiosis by influencing Plk1

• Published in: Theriogenology 2021-04, Vol.164, p.74-83
• Main Authors: Xu, Ying, Xu, Chang-Long, Xu, Zhong-Feng, Wang, Xin-Jie, Liang, Hui-Sheng, Zeng, Zhao-Cheng, Zeng, Li-Xin, Wei, Kang-Na, Deng, Shu-Zi, Xie, Shu-Juan, Jiang, Jiang, Liu, Yu-Xin, Cao, Yun-Kao, Wang, Hai-Long
• Format: Article
• Language: English
• Subjects: Adaptor Proteins, Signal Transducing; Animals; Cell Cycle Proteins - metabolism; Fbf1; Meiosis; Mice; Microtubules; Mouse; Nocodazole; Oocyte; Oocytes; Polo-Like Kinase 1; Protein Serine-Threonine Kinases - metabolism; Proto-Oncogene Proteins - metabolism; Spindle; Spindle Apparatus
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2021.01.018

Fas binding factor 1 (Fbf1) is one of the distal appendage proteins in the centriole, located at its distal and proximal ends. It influences the duplication and separation of centrosomes, thereby affecting the progression of the cell cycle during mitosis. However, the function of Fbf1 in meiosis has remained unclear. To explore the role of Fbf1 in the in vitro maturation of mouse oocyte, immunofluorescence staining was used to examine the Fbf1 location in the oocyte and their phenotype after protein deletion. Western blot was used to examine the protein abundance. This study showed that mouse oocytes express Fbf1 which locates at the spindle poles and around the microtubules. Through taxol and nocodazole treatment, and microinjection of siRNA, it was demonstrated that Fbf1 had an important role in the spindle assembly and chromosome separation during mouse oocyte meiosis In particular, microinjection of Fbf1-siRNA resulted in severe abnormalities in the spindle and chromosome arrangement, decreased aggregation of microtubules, disrupted the first oocyte meiosis, and the extrusion of the first polar body. Furthermore, in the Fbf1-siRNA group, there was reduced expression of Plk1 and its agglutination at the spindle poles, along with retarded chromosome segregation due to the activation of the spindle assembly checkpoint (SAC) component BubR1. These results indicate that Fbf1 may function in microtubule depolymerization and agglutination, control the microtubule dynamics, spindle assembly and chromosome arrangement and, thus, influence the mouse oocyte meiotic maturation. [Display omitted] •FBF1 was colocated with spindle microtubules at various stages of oocyte maturation.•FBF1 knockdown would cause abnormal spindle microtubule assembly, abnormal chromosome arrangement in the first meiosis.•Down-modulated FBF1 blocked the transition in the middle and late stages.•FBF1 knockdown would lead to the disappearance of PLK1 localization, and the protein level was significantly reduced.