High‐speed centrifugation of extender of freeze‐thaw boar semen

In semen cryopreservation, egg yolk is still widely used as a non‐penetrating cryoprotectant. Much has been developed in the search for alternatives for this biological product. This work aimed to evaluate the processed egg yolk through ultracentrifugation and/or sonication in the cryopreservation o...

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Bibliographic Details
Published in:Reproduction in domestic animals 2021-05, Vol.56 (5), p.821-825
Main Authors: Brito, Camila R. C., Varela Junior, Antonio Sergio, Gheller, Stela Mari M., Acosta, Izani B., Anciuti, Andreia N., Gatti, Norton Luiz S., Silva, Edenara A., Knabah, Nathalia W., Corcini, Carine D.
Format: Article
Language:English
Subjects:
boar
Centrifugation
Cryopreservation
Cryoprotectors
Eggs
Freeze-thawing
high‐speed centrifugation
kinematic parameters
Lactose
Mitochondria
Parameters
Semen
Sonication
Swine
Thawing
Ultracentrifugation
Yolk
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Summary:In semen cryopreservation, egg yolk is still widely used as a non‐penetrating cryoprotectant. Much has been developed in the search for alternatives for this biological product. This work aimed to evaluate the processed egg yolk through ultracentrifugation and/or sonication in the cryopreservation of swine semen. Twenty‐seven semen doses were purchased from a commercial boar stud and processed for cryopreservation using egg yolk lactose 11% (control) extender, processed using two different methods: high‐speed centrifugation and sonication. Then, they were submitted to freeze‐thawing protocol and were assessed for kinematic and cell structural parameters. Samples in which extenders underwent centrifugation had better results in velocity parameters, meanwhile those that only sonication was performed had poorest results in this parameter. The preservation of the membrane and mitochondria structure had better results when the diluent was only centrifuged in comparison with the other treatments. Therefore, centrifugation of extender containing egg yolk is important for better cryopreservation of swine semen.
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.13915