Janus kinase/signal transducer and activator of transcription signaling pathway-related genes STAT3, SOCS3 and their role in thiram induced tibial dyschondroplasia chickens

• Published in: Research in veterinary science 2021-05, Vol.136, p.25-31
• Main Authors: Chen, Shu-ming, Jahejo, Ali Raza, Nabi, Fazul, Ahmed, Shakeel, Zhao, Jin-feng, Yu, Jin, Zhang, Chen-liang, Ning, Guan-bao, Zhang, Ding, Raza, Sayed Haidar Abbas, Tian, Wen-xia
• Format: Article
• Language: English
• Subjects: Apoptosis; Cartilage; Cell cycle; Cell growth; Chicken; Chickens; Chondrocytes; Chondrogenesis; Gene expression; Genes; Growth plate; Histology; JAK/STAT; Janus kinase; Kinases; Localization; Lymphoma; Mineralization; Morphology; p-STAT3; Pathogenicity; Pathogens; Polyclonal antibodies; Poultry; Proteins; Signal transduction; Signaling; SOCS3; Software; Stat3 protein; Thiram; Transcription; Veterinary medicine
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2021.01.024

Pathogenicity of tibial dyschondroplasia (TD) in broiler chickens is not detected yet. Janus Kinase/Signal Transducer and Activator of Transcription (JAK-STAT) signaling pathway-related genes were investigated in thiram induced TD chickens. Real-time qPCR and immunohistochemical (IHC) technique were used to observe the expression changes of STAT3 and SOSC3 gene on days 1, 2, 4, 6 after feeding 100 mg·kg−1 thiram. Morphological, pathological, and histological results of this study suggested that chondrocyte cells were observed more damaged on day 6 than day 1, 2, and 4. Therefore, Lameness and damaged chondrocytes gradually increased from day 1 to 6. The mRNA expression level of STAT3 was observed insignificant (P > 0.05) in thiram induced TD chickens’ group of day 1. However, on days 2, 4, and 6, the expression was significant (P