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Janus kinase/signal transducer and activator of transcription signaling pathway-related genes STAT3, SOCS3 and their role in thiram induced tibial dyschondroplasia chickens
Pathogenicity of tibial dyschondroplasia (TD) in broiler chickens is not detected yet. Janus Kinase/Signal Transducer and Activator of Transcription (JAK-STAT) signaling pathway-related genes were investigated in thiram induced TD chickens. Real-time qPCR and immunohistochemical (IHC) technique were...
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| Published in: | Research in veterinary science 2021-05, Vol.136, p.25-31 |
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| creator | Chen, Shu-ming Jahejo, Ali Raza Nabi, Fazul Ahmed, Shakeel Zhao, Jin-feng Yu, Jin Zhang, Chen-liang Ning, Guan-bao Zhang, Ding Raza, Sayed Haidar Abbas Tian, Wen-xia |
| description | Pathogenicity of tibial dyschondroplasia (TD) in broiler chickens is not detected yet. Janus Kinase/Signal Transducer and Activator of Transcription (JAK-STAT) signaling pathway-related genes were investigated in thiram induced TD chickens.
Real-time qPCR and immunohistochemical (IHC) technique were used to observe the expression changes of STAT3 and SOSC3 gene on days 1, 2, 4, 6 after feeding 100 mg·kg−1 thiram.
Morphological, pathological, and histological results of this study suggested that chondrocyte cells were observed more damaged on day 6 than day 1, 2, and 4. Therefore, Lameness and damaged chondrocytes gradually increased from day 1 to 6.
The mRNA expression level of STAT3 was observed insignificant (P > 0.05) in thiram induced TD chickens’ group of day 1. However, on days 2, 4, and 6, the expression was significant (P |
| doi_str_mv | 10.1016/j.rvsc.2021.01.024 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2489256815</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0034528821000369</els_id><sourcerecordid>2527534881</sourcerecordid><originalsourceid>FETCH-LOGICAL-c384t-8458f8528351f6445e42bc755273bc1af2b5eb1dba14aa3f4845832c89db3f383</originalsourceid><addsrcrecordid>eNp9UcuO0zAUtRCIKYUfYIEssZkF6fiZuhKbUcVTI82iZW05zk3rTmoX2ynqP_GROGRgwQLpSrZ1z0M-B6HXlCwoofXNYRHPyS4YYXRByjDxBM2o5KxidU2fohkhXFSSKXWFXqR0IIQISpfP0RXncqnYis7Qz6_GDwk_OG8S3CS386bHORqf2sFCxMa32NjsziaHiEM37Wx0p-yCxxPB-R0-mbz_YS5VhN5kaPEOPCS82d5u-Tu8uV9v-G-tvAcXcQw9YOfLy0VzLLfRrCxd44p9e0l2H3wbw6k3yRls984-gE8v0bPO9AlePZ5z9O3jh-36c3V3_-nL-vauslyJXCkhVafKv7mkXS2EBMEau5SSLXljqelYI6GhbWOoMIZ3YiRwZtWqbXjHFZ-j60n3FMP3AVLWR5cs9L3xEIakmVArJmtVsp6jt_9AD2GIJZOCKn6SC6VoQbEJZWNIKUKnT9EdTbxoSvTYpT7osUs9dqlJGSYK6c2j9NAcof1L-VNeAbyfAFCyODuIOlkHviTpItis2-D-p_8LyBKyag</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2527534881</pqid></control><display><type>article</type><title>Janus kinase/signal transducer and activator of transcription signaling pathway-related genes STAT3, SOCS3 and their role in thiram induced tibial dyschondroplasia chickens</title><source>ScienceDirect Journals</source><creator>Chen, Shu-ming ; Jahejo, Ali Raza ; Nabi, Fazul ; Ahmed, Shakeel ; Zhao, Jin-feng ; Yu, Jin ; Zhang, Chen-liang ; Ning, Guan-bao ; Zhang, Ding ; Raza, Sayed Haidar Abbas ; Tian, Wen-xia</creator><creatorcontrib>Chen, Shu-ming ; Jahejo, Ali Raza ; Nabi, Fazul ; Ahmed, Shakeel ; Zhao, Jin-feng ; Yu, Jin ; Zhang, Chen-liang ; Ning, Guan-bao ; Zhang, Ding ; Raza, Sayed Haidar Abbas ; Tian, Wen-xia</creatorcontrib><description>Pathogenicity of tibial dyschondroplasia (TD) in broiler chickens is not detected yet. Janus Kinase/Signal Transducer and Activator of Transcription (JAK-STAT) signaling pathway-related genes were investigated in thiram induced TD chickens.
Real-time qPCR and immunohistochemical (IHC) technique were used to observe the expression changes of STAT3 and SOSC3 gene on days 1, 2, 4, 6 after feeding 100 mg·kg−1 thiram.
Morphological, pathological, and histological results of this study suggested that chondrocyte cells were observed more damaged on day 6 than day 1, 2, and 4. Therefore, Lameness and damaged chondrocytes gradually increased from day 1 to 6.
The mRNA expression level of STAT3 was observed insignificant (P > 0.05) in thiram induced TD chickens’ group of day 1. However, on days 2, 4, and 6, the expression was significant (P < 0.05). SOCS3 increased in thiram group on days 1, 2 and 6, decreased on day 4 (P < 0.05). The p-STAT3 and SOCS3 protein's protein localization was evaluated in the control and thiram-induced TD broiler chickens through IHC, suggesting that SOSC3 protein was observed significantly higher on days 1, 2, and 6 and down-regulated on day 4. p-STAT3 protein on thiram induced group was observed significantly upregulated on days 4 and 6.
In conclusion, the differential expression of STAT3 and SOCS3 showed that the JAK-STAT signaling pathway might play an important role in regulating an abnormal proliferation, differentiation, or apoptosis of chondrocytes in TD at an early stage.
JAK-STAT signaling pathway and related-genes (STAT3 and SOSC3) have an important role in chondrocytes proliferation and apoptosis. [Display omitted]
•JAK-STAT signaling pathway investigated in thiram induced TD chickens.•The expression level of STAT3 and SOCS3 genes initially increased and then decreased.•p-STAT3 and SOCS3 were expressed in PZ, PH, and HZ of the growth plate.•p-STAT3 and SOCS3 showed that the JAK-STAT signaling pathway might play an important role in TD.•Thiram may regulate the proliferation, apoptosis of chondrocytes through the JAK-STAT signaling pathway.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2021.01.024</identifier><identifier>PMID: 33578291</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Apoptosis ; Cartilage ; Cell cycle ; Cell growth ; Chicken ; Chickens ; Chondrocytes ; Chondrogenesis ; Gene expression ; Genes ; Growth plate ; Histology ; JAK/STAT ; Janus kinase ; Kinases ; Localization ; Lymphoma ; Mineralization ; Morphology ; p-STAT3 ; Pathogenicity ; Pathogens ; Polyclonal antibodies ; Poultry ; Proteins ; Signal transduction ; Signaling ; SOCS3 ; Software ; Stat3 protein ; Thiram ; Transcription ; Veterinary medicine</subject><ispartof>Research in veterinary science, 2021-05, Vol.136, p.25-31</ispartof><rights>2021 Elsevier Ltd</rights><rights>Copyright © 2021 Elsevier Ltd. All rights reserved.</rights><rights>2021. Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-8458f8528351f6445e42bc755273bc1af2b5eb1dba14aa3f4845832c89db3f383</citedby><cites>FETCH-LOGICAL-c384t-8458f8528351f6445e42bc755273bc1af2b5eb1dba14aa3f4845832c89db3f383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33578291$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Shu-ming</creatorcontrib><creatorcontrib>Jahejo, Ali Raza</creatorcontrib><creatorcontrib>Nabi, Fazul</creatorcontrib><creatorcontrib>Ahmed, Shakeel</creatorcontrib><creatorcontrib>Zhao, Jin-feng</creatorcontrib><creatorcontrib>Yu, Jin</creatorcontrib><creatorcontrib>Zhang, Chen-liang</creatorcontrib><creatorcontrib>Ning, Guan-bao</creatorcontrib><creatorcontrib>Zhang, Ding</creatorcontrib><creatorcontrib>Raza, Sayed Haidar Abbas</creatorcontrib><creatorcontrib>Tian, Wen-xia</creatorcontrib><title>Janus kinase/signal transducer and activator of transcription signaling pathway-related genes STAT3, SOCS3 and their role in thiram induced tibial dyschondroplasia chickens</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>Pathogenicity of tibial dyschondroplasia (TD) in broiler chickens is not detected yet. Janus Kinase/Signal Transducer and Activator of Transcription (JAK-STAT) signaling pathway-related genes were investigated in thiram induced TD chickens.
Real-time qPCR and immunohistochemical (IHC) technique were used to observe the expression changes of STAT3 and SOSC3 gene on days 1, 2, 4, 6 after feeding 100 mg·kg−1 thiram.
Morphological, pathological, and histological results of this study suggested that chondrocyte cells were observed more damaged on day 6 than day 1, 2, and 4. Therefore, Lameness and damaged chondrocytes gradually increased from day 1 to 6.
The mRNA expression level of STAT3 was observed insignificant (P > 0.05) in thiram induced TD chickens’ group of day 1. However, on days 2, 4, and 6, the expression was significant (P < 0.05). SOCS3 increased in thiram group on days 1, 2 and 6, decreased on day 4 (P < 0.05). The p-STAT3 and SOCS3 protein's protein localization was evaluated in the control and thiram-induced TD broiler chickens through IHC, suggesting that SOSC3 protein was observed significantly higher on days 1, 2, and 6 and down-regulated on day 4. p-STAT3 protein on thiram induced group was observed significantly upregulated on days 4 and 6.
In conclusion, the differential expression of STAT3 and SOCS3 showed that the JAK-STAT signaling pathway might play an important role in regulating an abnormal proliferation, differentiation, or apoptosis of chondrocytes in TD at an early stage.
JAK-STAT signaling pathway and related-genes (STAT3 and SOSC3) have an important role in chondrocytes proliferation and apoptosis. [Display omitted]
•JAK-STAT signaling pathway investigated in thiram induced TD chickens.•The expression level of STAT3 and SOCS3 genes initially increased and then decreased.•p-STAT3 and SOCS3 were expressed in PZ, PH, and HZ of the growth plate.•p-STAT3 and SOCS3 showed that the JAK-STAT signaling pathway might play an important role in TD.•Thiram may regulate the proliferation, apoptosis of chondrocytes through the JAK-STAT signaling pathway.</description><subject>Apoptosis</subject><subject>Cartilage</subject><subject>Cell cycle</subject><subject>Cell growth</subject><subject>Chicken</subject><subject>Chickens</subject><subject>Chondrocytes</subject><subject>Chondrogenesis</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Growth plate</subject><subject>Histology</subject><subject>JAK/STAT</subject><subject>Janus kinase</subject><subject>Kinases</subject><subject>Localization</subject><subject>Lymphoma</subject><subject>Mineralization</subject><subject>Morphology</subject><subject>p-STAT3</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Polyclonal antibodies</subject><subject>Poultry</subject><subject>Proteins</subject><subject>Signal transduction</subject><subject>Signaling</subject><subject>SOCS3</subject><subject>Software</subject><subject>Stat3 protein</subject><subject>Thiram</subject><subject>Transcription</subject><subject>Veterinary medicine</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9UcuO0zAUtRCIKYUfYIEssZkF6fiZuhKbUcVTI82iZW05zk3rTmoX2ynqP_GROGRgwQLpSrZ1z0M-B6HXlCwoofXNYRHPyS4YYXRByjDxBM2o5KxidU2fohkhXFSSKXWFXqR0IIQISpfP0RXncqnYis7Qz6_GDwk_OG8S3CS386bHORqf2sFCxMa32NjsziaHiEM37Wx0p-yCxxPB-R0-mbz_YS5VhN5kaPEOPCS82d5u-Tu8uV9v-G-tvAcXcQw9YOfLy0VzLLfRrCxd44p9e0l2H3wbw6k3yRls984-gE8v0bPO9AlePZ5z9O3jh-36c3V3_-nL-vauslyJXCkhVafKv7mkXS2EBMEau5SSLXljqelYI6GhbWOoMIZ3YiRwZtWqbXjHFZ-j60n3FMP3AVLWR5cs9L3xEIakmVArJmtVsp6jt_9AD2GIJZOCKn6SC6VoQbEJZWNIKUKnT9EdTbxoSvTYpT7osUs9dqlJGSYK6c2j9NAcof1L-VNeAbyfAFCyODuIOlkHviTpItis2-D-p_8LyBKyag</recordid><startdate>202105</startdate><enddate>202105</enddate><creator>Chen, Shu-ming</creator><creator>Jahejo, Ali Raza</creator><creator>Nabi, Fazul</creator><creator>Ahmed, Shakeel</creator><creator>Zhao, Jin-feng</creator><creator>Yu, Jin</creator><creator>Zhang, Chen-liang</creator><creator>Ning, Guan-bao</creator><creator>Zhang, Ding</creator><creator>Raza, Sayed Haidar Abbas</creator><creator>Tian, Wen-xia</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>202105</creationdate><title>Janus kinase/signal transducer and activator of transcription signaling pathway-related genes STAT3, SOCS3 and their role in thiram induced tibial dyschondroplasia chickens</title><author>Chen, Shu-ming ; Jahejo, Ali Raza ; Nabi, Fazul ; Ahmed, Shakeel ; Zhao, Jin-feng ; Yu, Jin ; Zhang, Chen-liang ; Ning, Guan-bao ; Zhang, Ding ; Raza, Sayed Haidar Abbas ; Tian, Wen-xia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-8458f8528351f6445e42bc755273bc1af2b5eb1dba14aa3f4845832c89db3f383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Apoptosis</topic><topic>Cartilage</topic><topic>Cell cycle</topic><topic>Cell growth</topic><topic>Chicken</topic><topic>Chickens</topic><topic>Chondrocytes</topic><topic>Chondrogenesis</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Growth plate</topic><topic>Histology</topic><topic>JAK/STAT</topic><topic>Janus kinase</topic><topic>Kinases</topic><topic>Localization</topic><topic>Lymphoma</topic><topic>Mineralization</topic><topic>Morphology</topic><topic>p-STAT3</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Polyclonal antibodies</topic><topic>Poultry</topic><topic>Proteins</topic><topic>Signal transduction</topic><topic>Signaling</topic><topic>SOCS3</topic><topic>Software</topic><topic>Stat3 protein</topic><topic>Thiram</topic><topic>Transcription</topic><topic>Veterinary medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Shu-ming</creatorcontrib><creatorcontrib>Jahejo, Ali Raza</creatorcontrib><creatorcontrib>Nabi, Fazul</creatorcontrib><creatorcontrib>Ahmed, Shakeel</creatorcontrib><creatorcontrib>Zhao, Jin-feng</creatorcontrib><creatorcontrib>Yu, Jin</creatorcontrib><creatorcontrib>Zhang, Chen-liang</creatorcontrib><creatorcontrib>Ning, Guan-bao</creatorcontrib><creatorcontrib>Zhang, Ding</creatorcontrib><creatorcontrib>Raza, Sayed Haidar Abbas</creatorcontrib><creatorcontrib>Tian, Wen-xia</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Shu-ming</au><au>Jahejo, Ali Raza</au><au>Nabi, Fazul</au><au>Ahmed, Shakeel</au><au>Zhao, Jin-feng</au><au>Yu, Jin</au><au>Zhang, Chen-liang</au><au>Ning, Guan-bao</au><au>Zhang, Ding</au><au>Raza, Sayed Haidar Abbas</au><au>Tian, Wen-xia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Janus kinase/signal transducer and activator of transcription signaling pathway-related genes STAT3, SOCS3 and their role in thiram induced tibial dyschondroplasia chickens</atitle><jtitle>Research in veterinary science</jtitle><addtitle>Res Vet Sci</addtitle><date>2021-05</date><risdate>2021</risdate><volume>136</volume><spage>25</spage><epage>31</epage><pages>25-31</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>Pathogenicity of tibial dyschondroplasia (TD) in broiler chickens is not detected yet. Janus Kinase/Signal Transducer and Activator of Transcription (JAK-STAT) signaling pathway-related genes were investigated in thiram induced TD chickens.
Real-time qPCR and immunohistochemical (IHC) technique were used to observe the expression changes of STAT3 and SOSC3 gene on days 1, 2, 4, 6 after feeding 100 mg·kg−1 thiram.
Morphological, pathological, and histological results of this study suggested that chondrocyte cells were observed more damaged on day 6 than day 1, 2, and 4. Therefore, Lameness and damaged chondrocytes gradually increased from day 1 to 6.
The mRNA expression level of STAT3 was observed insignificant (P > 0.05) in thiram induced TD chickens’ group of day 1. However, on days 2, 4, and 6, the expression was significant (P < 0.05). SOCS3 increased in thiram group on days 1, 2 and 6, decreased on day 4 (P < 0.05). The p-STAT3 and SOCS3 protein's protein localization was evaluated in the control and thiram-induced TD broiler chickens through IHC, suggesting that SOSC3 protein was observed significantly higher on days 1, 2, and 6 and down-regulated on day 4. p-STAT3 protein on thiram induced group was observed significantly upregulated on days 4 and 6.
In conclusion, the differential expression of STAT3 and SOCS3 showed that the JAK-STAT signaling pathway might play an important role in regulating an abnormal proliferation, differentiation, or apoptosis of chondrocytes in TD at an early stage.
JAK-STAT signaling pathway and related-genes (STAT3 and SOSC3) have an important role in chondrocytes proliferation and apoptosis. [Display omitted]
•JAK-STAT signaling pathway investigated in thiram induced TD chickens.•The expression level of STAT3 and SOCS3 genes initially increased and then decreased.•p-STAT3 and SOCS3 were expressed in PZ, PH, and HZ of the growth plate.•p-STAT3 and SOCS3 showed that the JAK-STAT signaling pathway might play an important role in TD.•Thiram may regulate the proliferation, apoptosis of chondrocytes through the JAK-STAT signaling pathway.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>33578291</pmid><doi>10.1016/j.rvsc.2021.01.024</doi><tpages>7</tpages></addata></record> |
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| source | ScienceDirect Journals |
| subjects | Apoptosis Cartilage Cell cycle Cell growth Chicken Chickens Chondrocytes Chondrogenesis Gene expression Genes Growth plate Histology JAK/STAT Janus kinase Kinases Localization Lymphoma Mineralization Morphology p-STAT3 Pathogenicity Pathogens Polyclonal antibodies Poultry Proteins Signal transduction Signaling SOCS3 Software Stat3 protein Thiram Transcription Veterinary medicine |
| title | Janus kinase/signal transducer and activator of transcription signaling pathway-related genes STAT3, SOCS3 and their role in thiram induced tibial dyschondroplasia chickens |
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