Clonal dispersion of Acinetobacter baumannii in an intensive care unit designed to patients COVID-19

• Published in: Journal of infection in developing countries 2021-01, Vol.15 (1), p.58-68
• Main Authors: Durán-Manuel, Emilio Mariano, Cruz-Cruz, Clemente, Ibáñez-Cervantes, Gabriela, Bravata-Alcantará, Juan Carlos, Sosa-Hernández, Oscar, Delgado-Balbuena, Laura, León-García, Gregorio, Cortés-Ortíz, Iliana Alejandra, Cureño-Díaz, Monica Alethia, Castro-Escarpulli, Graciela, Vélez-Reséndiz, Juan Manuel, Bello-López, Juan Manuel
• Format: Article
• Language: English
• Subjects: Acinetobacter baumannii - isolation & purification; Acinetobacter baumannii - pathogenicity; Acinetobacter Infections - transmission; Anti-Bacterial Agents - pharmacology; Bacteria; Biofilms - growth & development; Coronaviruses; COVID-19; COVID-19 - prevention & control; Cross Infection - microbiology; Cross Infection - prevention & control; Drug Resistance, Bacterial - genetics; Equipment and Supplies - microbiology; Genotype; Humans; Intensive care; Intensive Care Units; Interspersed Repetitive Sequences; Medical equipment; Medical personnel; Mexico; Pneumonia, Ventilator-Associated - microbiology
• ISSN: 1972-2680; 2036-6590; 1972-2680
• DOI: 10.3855/jidc.13545

SARS-CoV2 pandemic marks the need to pay attention to bacterial pathogens that can complicate the hospital stay of patients in the intensive care unit (ICU). ESKAPE bacteria which includes Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter cloacae are considered the most important, because of their close relationship with the development of ventilator-associated pneumonia (VAP). The aim of this work was to identify and characterize ESKAPE bacteria and to detect their possible clonal spread in medical devices, patients, and medical personnel of the ICU for COVID-19 patients of the Hospital Juarez de Mexico. Genetic identification of ESKAPE bacteria was performed by analyzing the 16S rRNA gene. Resistance assays were performed according to the CLSI guidelines. Assembly of AdeABCRS operon and inhibition assays of pumps efflux in Acinetobacter baumannii isolates were performed. Associated gene involved in biofilm formation (icaA) was performed in isolates belonging to the Staphylococcus genus. Finally, typing by ERIC-PCR and characterization of mobile genetic element SCCmec were done. Heterogeneous distribution of ESKAPE and non-ESKAPE bacteria was detected in various medical devices, patients, and medical personnel. Acinetobacter baumannii and Staphylococcus aureus were the predominant ESKAPE members. The analysis of intergenic regions revealed an important clonal distribution of A. baumannii (AdeABCRS+). Genotyping of SCCmec mobile genetic elements and the icaA gene showed that there is no clonal distribution of S. aureus. Clonal spread of A. baumannii (AdeABCRS+) highlights the importance of adopting good practices for equipment disinfection, surfaces and management of COVID-19 patients.