Inhibitory effects of cyanidin-3-O-glucoside in black soybean hull extract on RBL-2H3 cells degranulation and passive cutaneous anaphylaxis reaction in mice

[Display omitted] •BSHE inhibits the IgE-mediated degranulation in RBL-2H3 cells.•One of the active compounds involved in the degranulation of BSHE is C3G.•Oral administering of C3G to IgE-sensitized mice suppresses the PCA reaction.•BSHE and C3G could exert for the prevention or the treatment of ty...

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Bibliographic Details
Published in:International immunopharmacology 2021-05, Vol.94, p.107394-107394, Article 107394
Main Authors: Hiemori-Kondo, Miki, Morikawa, Etsuko, Fujikura, Michiko, Nagayasu, Ayuko, Maekawa, Yuuki
Format: Article
Language:English
Subjects:
Allergic reactions
Allergies
Anaphylaxis
Animals
Anthocyanins
Anthocyanins - pharmacology
Anthocyanins - therapeutic use
Anti-allergy
Antigens
Basophilic leukemia
Black soybean hull extract
Cell Degranulation - drug effects
Cell Line
Cyanidin-3-O-glucoside
Degranulation
Dosage
Drug development
Formulations
Glucosides
Glycine max
Hexosaminidases - metabolism
Histamine
Histamine Release - drug effects
Immunoglobulin E
Intravenous administration
Ionization
Leukemia
Liquid chromatography
Male
Mass spectrometry
Mass spectroscopy
Mice, Inbred ICR
Oral administration
Passive cutaneous anaphylaxis
Passive Cutaneous Anaphylaxis - drug effects
Passive cutaneous anaphylaxis reaction
Plant Extracts
Rats
RBL-2H3 cells
Soybeans
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Summary:[Display omitted] •BSHE inhibits the IgE-mediated degranulation in RBL-2H3 cells.•One of the active compounds involved in the degranulation of BSHE is C3G.•Oral administering of C3G to IgE-sensitized mice suppresses the PCA reaction.•BSHE and C3G could exert for the prevention or the treatment of type I allergies. Black soybean hull extract (BSHE) exhibits a variety of biological activities. However, little is known about the effects of BSHE on immunoglobulin E (IgE)-mediated type I allergic reactions. The anti-allergic effect of BSHE was assessed with the degranulation assay using rat basophilic leukemia RBL-2H3 cells and the passive cutaneous anaphylaxis (PCA) reaction in mice. An active compound in BSHE was identified by ultra-performance liquid chromatography coupled to diode array detection and electrospray ionization tandem mass spectrometry analysis. BSHE inhibited the release of β-hexosaminidase and histamine in RBL-2H3 cells, and cyanidin-3-O-glucoside (C3G) was identified as one of its active compounds. Oral administering of 200 μmol/kg of C3G to IgE-sensitized mice prior to antigen injection suppressed the PCA reaction, as compared with control (p 
ISSN:1567-5769
1878-1705
DOI:10.1016/j.intimp.2021.107394