Expression of α2-Na/K-ATPase in C57BL/6J Mice Inner Ear and Its Relationship with Age-related Hearing Loss

• Published in: Current medical science 2021-02, Vol.41 (1), p.153-157
• Main Authors: Liu, Yun, Chu, Han-qi, Sun, Yan-bo, Bing, Dan, Zhou, Liang-qiang, Chen, Jin, Chen, Qing-guo, Du, Zhi-hui
• Format: Article
• Language: English
• Subjects: Medicine; Medicine & Public Health
• ISSN: 2096-5230; 2523-899X
• DOI: 10.1007/s11596-021-2330-5

Summary K + cycling in the cochlea is critical to maintain hearing. Many sodium-potassium pumps are proved to participate in K + cycling, such as Na/K-ATPase. The α2-Na/K-ATPase is an important isoform of Na/K-ATPase. The expression of α2-Na/K-ATPase in the cochlea is not clear. In this study, we used C57BL/6 mice as a model of presbycusis and implemented immunohistochemistry staining and quantitative real time-PCR, and the α2-Na/K-ATPase expression pattern was confirmed in the inner ear. It was found α2-Na/K-ATPase was expressed widely in cochlea and its mRNA and protein expression was gradually reduced with aging (4-, 14-, 26- and 48-weeks old mice). We suspected that, the down-regulation of α2-Na/K-ATPase expression might be associated with the remodeling of K + cycling, degeneration of morphological structure and decrease of hearing function in aging C57 mice. In conclusion, we speculated that the reduction of α2-Na/K-ATPase might play an important role in the pathogenesis of age-related hearing loss.