Expression of α2-Na/K-ATPase in C57BL/6J Mice Inner Ear and Its Relationship with Age-related Hearing Loss

Summary K + cycling in the cochlea is critical to maintain hearing. Many sodium-potassium pumps are proved to participate in K + cycling, such as Na/K-ATPase. The α2-Na/K-ATPase is an important isoform of Na/K-ATPase. The expression of α2-Na/K-ATPase in the cochlea is not clear. In this study, we us...

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Published in:Current medical science 2021-02, Vol.41 (1), p.153-157
Main Authors: Liu, Yun, Chu, Han-qi, Sun, Yan-bo, Bing, Dan, Zhou, Liang-qiang, Chen, Jin, Chen, Qing-guo, Du, Zhi-hui
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cited_by cdi_FETCH-LOGICAL-c2952-5e31744c16bec731ac49a8ced47502d34567b7d405f9618d206f1186042d5083
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description Summary K + cycling in the cochlea is critical to maintain hearing. Many sodium-potassium pumps are proved to participate in K + cycling, such as Na/K-ATPase. The α2-Na/K-ATPase is an important isoform of Na/K-ATPase. The expression of α2-Na/K-ATPase in the cochlea is not clear. In this study, we used C57BL/6 mice as a model of presbycusis and implemented immunohistochemistry staining and quantitative real time-PCR, and the α2-Na/K-ATPase expression pattern was confirmed in the inner ear. It was found α2-Na/K-ATPase was expressed widely in cochlea and its mRNA and protein expression was gradually reduced with aging (4-, 14-, 26- and 48-weeks old mice). We suspected that, the down-regulation of α2-Na/K-ATPase expression might be associated with the remodeling of K + cycling, degeneration of morphological structure and decrease of hearing function in aging C57 mice. In conclusion, we speculated that the reduction of α2-Na/K-ATPase might play an important role in the pathogenesis of age-related hearing loss.
doi_str_mv 10.1007/s11596-021-2330-5
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Many sodium-potassium pumps are proved to participate in K + cycling, such as Na/K-ATPase. The α2-Na/K-ATPase is an important isoform of Na/K-ATPase. The expression of α2-Na/K-ATPase in the cochlea is not clear. In this study, we used C57BL/6 mice as a model of presbycusis and implemented immunohistochemistry staining and quantitative real time-PCR, and the α2-Na/K-ATPase expression pattern was confirmed in the inner ear. It was found α2-Na/K-ATPase was expressed widely in cochlea and its mRNA and protein expression was gradually reduced with aging (4-, 14-, 26- and 48-weeks old mice). We suspected that, the down-regulation of α2-Na/K-ATPase expression might be associated with the remodeling of K + cycling, degeneration of morphological structure and decrease of hearing function in aging C57 mice. 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title Expression of α2-Na/K-ATPase in C57BL/6J Mice Inner Ear and Its Relationship with Age-related Hearing Loss
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