Construction of novel antimicrobial peptide-modified extracellular matrix biologic scaffold material

• Published in: Biochemical and biophysical research communications 2021-03, Vol.546, p.162-168
• Main Authors: Liang, Changyan, Chen, Junlin, Zhang, Yu, Wei, Feng, Ling, You, Li, Xiaomao
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - metabolism; Antibacterial scaffold; Antimicrobial peptide; Cell Adhesion; Cell Proliferation; Chitosan; Chitosan - chemistry; Chitosan - metabolism; Drug Resistance, Microbial; Extracellular matrix; Extracellular Matrix - chemistry; Extracellular Matrix - metabolism; Female; Microbial Sensitivity Tests; Peptides - chemistry; Peptides - metabolism; Rabbits; Surface Properties; Tissue repair; Tissue Scaffolds - chemistry; Wound Healing
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2021.02.002

In the field of implantable medical devices, the antibacterial extracellular matrix (ECM) biologic scaffold, which is constructed by modifying biomaterials with antibacterial peptides, has excellent potential. An antibacterial peptide–modified ECM scaffold was formed with chitosan (CS), antimicrobial peptide (AMP), and ECM scaffold. Chitosan has a firm positive-charge surface and can combine with the ECM scaffold material to form a positive-charge layer on the surface. The surface potential was characterized using a surface potential map. Infrared spectroscopy and scanning electron microscopy (SEM) were used to observe the scaffold surface characteristics and cell morphology. Fluorescence staining and MTS assay kit were used to assess cytotoxicity and biocompatibility. To evaluate the antibacterial and repairing effects on the infected wounds in vivo, a subcutaneous antibacterial test of rabbit back was conducted. The antibacterial peptide–modified ECM scaffold was successfully formed and presented an excellent three-dimensional micro-surface porous structure. The antibacterial peptide–modified ECM scaffold could be effectively-prepared by surface modification and activation. Fluorescence staining tests showed good cell adhesion, proliferation ability, and cell affinity. The in vivo experiment indicated that the antibacterial ECM scaffold had antibacterial and healing-promotion abilities. •Solution to the problem of antibiotic resistance in extracellular matrix scaffold implantation.•Surface features of antimicrobial peptide modified extracellular matrix scaffold.•A potential material for tissue damage repair and indicated a new research thought for the extracellular matrix scaffold.•Extracellular matrix scaffold coated with different concentrations of CS present various biocompatibility.