Influence of faecal culture media and incubation time on the yield of infective larvae of Haemonchus contortus (Rudolphi 1803)

• Published in: Parasitology research (1987) 2021-04, Vol.120 (4), p.1493-1497
• Main Authors: Muchiut, Sebastián, Fernández, Silvina, Domínguez, Paula, Riva, Eliana, Rodríguez, Edgardo, Steffan, Pedro, Bernat, Gisele, Fiel, César
• Format: Article
• Language: English
• Subjects: Additives; Biomedical and Life Sciences; Biomedicine; Culture media; Feces; Haemonchus contortus; Helminthology - Short Communication; Immunology; Medical Microbiology; Microbiology; Polystyrene
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-021-07078-y

The aim of this experiment was to determine the yield of Haemonchus contortus third-stage larvae (L3) in faecal cultures in different conditions, including incubation time (7 or 14 days), the addition of inert additives (polystyrene pellets, vermiculite or no additive) and physical condition of the incubated faeces (ground or whole pellets). Twelve groups of 10 cultures each were arranged and incubated at 24 °C to evaluate the interaction of the above-mentioned conditions. Significantly, more L3 ( p =0.0019 to p =0.0200) were recovered from cultures incubated for 7 days than for 14 days, except for the groups containing whole pellets with no additives ( p =0.53) or with vermiculite ( p =0.41). Larval yields from 7-day incubated cultures did not differ between groups ( p =0.47), but for the whole pellets with vermiculite group, which yielded significantly less L3 ( p