Isolation and characterization of a novel antimicrobial oxatetracyclo ketone from Bacillus stercoris MBTDCMFRI Ba37 isolated from the tropical estuarine habitats of Cochin

• Published in: Molecular biology reports 2021-02, Vol.48 (2), p.1299-1310
• Main Authors: Nair, Anusree V., Praveen, N. K., Joseph, Neetha, Leo, Antony M., Vijayan, K. K.
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Anti-Infective Agents - chemistry; Anti-Infective Agents - isolation & purification; Anti-Infective Agents - pharmacology; Antibacterial activity; Aquaculture; Bacillus; Bacillus - chemistry; Biological control; Biomedical and Life Sciences; Cell culture; Culture Media - chemistry; Culture Media - pharmacology; Ecosystem; Glutamic acid; Glycerol; Histology; Ketones - chemistry; Ketones - isolation & purification; Ketones - pharmacology; Life Sciences; Microbial Sensitivity Tests; Morphology; Original Article; Pathogens; pH effects; Probiotics - chemistry; Probiotics - pharmacology; Proteolysis; Proteolytic enzymes; Vibrio - drug effects; Vibrio - pathogenicity
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-021-06146-x

Antimicrobial compounds from the safest source have gained greater relevance because of their wide spectrum of possible applications, especially in aquaculture industry, where pathogenic threat and antibacterial resistance are serious concerns. Bacillus stercoris MBTDCMFRI Ba37 isolated from mangrove environment of tropical estuarine habitats of Cochin exhibited a wide spectrum of antibacterial activity against major aquaculture pathogens belonging to genus Vibrio and Aeromonas. The structural characterization of the antibacterial compound from this strain may help in identifying their role as a biocontrol agent in aquaculture and allied sectors. The highest antibacterial activity was detected in 3rd day culture, grown in a modified Bacillus medium containing 1% of glycerol and 0.5% of glutamic acid at 30 °C, pH 8.0 and 15 ppt saline conditions. The inhibitory activity of the cell free supernatant was evident even at 20% v/v dilution. Preliminary studies on the nature of antibacterial action indicated that the bioactive principle is stable at temperatures up to 70 °C, between pH 6–9 and instable to lyzozyme and proteolytic enzymes. Bioassay guided purification followed by spectroscopic characterization of active fractions of B. stercoris MBTDCMFRI Ba37 revealed that the compound 1-(1-Hydroxyethyl)-1,7,10,12,13,15,17 heptamethyl-16-oxatetracyclo[8.7.0.0 2,3 .0 12,13 ]heptadecan-5-one, is responsible for its major antibacterial activity. This is the first report on isolation and characterization of an antibacterial compound from the species B. stercoris . The results of this study indicated that B. stercoris MBTDCMFRI Ba37 has beneficial antibacterial properties which could be useful in developing novel antimicrobial therapeutics against a variety of aquaculture and other pathogens.