A novel methymycin analog, 12-ketomethymycin N-oxide, produced by the heterologous expression of the large pikromycin/methymycin biosynthetic gene cluster of Streptomyces sp. AM4900

ABSTRACT A novel methymycin analog, 12-ketomethymycin N-oxide, was produced by the heterologous expression of the pikromycin/methymycin biosynthetic gene cluster of Streptomyces sp. AM4900 together with 12-ketomethymycin, which was only isolated by the biotransformation of the synthetic intermediate...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2021-03, Vol.85 (4), p.890-894
Main Authors: Ueoka, Reiko, Hashimoto, Junko, Kozone, Ikuko, Hashimoto, Takuya, Kudo, Kei, Kagaya, Noritaka, Suenaga, Hikaru, Ikeda, Haruo, Shin-ya, Kazuo
Format: Article
Language:English
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Summary:ABSTRACT A novel methymycin analog, 12-ketomethymycin N-oxide, was produced by the heterologous expression of the pikromycin/methymycin biosynthetic gene cluster of Streptomyces sp. AM4900 together with 12-ketomethymycin, which was only isolated by the biotransformation of the synthetic intermediate before. Their structures were determined by the spectroscopic data and the chemical derivatization. 12-Ketomethymycin showed a weak cytotoxicity against SKOV-3 and Jurkat cells, although its N-oxide analog did not show any activity. Both showed no antibacterial activities against Escherichia coli and Micrococcus luteus. Graphical Abstract Graphical Abstract 12-Ketomethymycin N-oxide (1).
ISSN:1347-6947
1347-6947
DOI:10.1093/bbb/zbaa111