Possible mechanisms for the renoprotective action of adipose-derived mesenchymal stem cells with CD44-targeted hyaluronic acid against renal ischemia

• Published in: Life sciences (1973) 2021-05, Vol.272, p.119221-119221, Article 119221
• Main Authors: Awadalla, Amira, Hussein, Abdelaziz M., Ali, Mohamed, Barakat, Nashwa, Hamam, Eman T., Magar, Raghda W., Shokeir, Ahmed A.
• Format: Article
• Language: English
• Subjects: Adipose mesenchymal stem cells; Adipose Tissue - metabolism; Angiogenesis; Animals; Apoptosis; CD44 antigen; Collagen (type I); Creatinine; Fibronectin; Fibrosis; Glutathione; Growth factors; Hyaluronic acid; Hyaluronic Acid - metabolism; Hyaluronic Acid - pharmacology; Inflammation - metabolism; Ischemia; Ischemia - metabolism; Ischemia - therapy; Kidney - pathology; Kidney Diseases - metabolism; Kidneys; Male; Malondialdehyde; Mesenchymal Stem Cell Transplantation - methods; Mesenchymal stem cells; Mesenchymal Stem Cells - cytology; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; Nitric oxide; Nitric-oxide synthase; Oxidative stress; Oxidative Stress - physiology; Parameters; Preconditioning; Rats; Rats, Sprague-Dawley; Renal ischemia/reperfusion injury; Reperfusion Injury - metabolism; Stem cell transplantation; Stem cells; Superoxide dismutase; Transforming growth factor-b1; Vascular endothelial growth factor; Wnt protein; β-Catenin
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2021.119221

The present study aimed to investigate the invitro preconditioning of adipose-derived mesenchymal stem cells (ADMSCs) with CD44-targeted hyalournic acid (HA) on ischemic kidney injury in rats. Ninety male Sprague Dawley rats were randomly allocated into the following groups; i) sham group, ii) control group: rats exposed to 45 min left renal ischemia with saline treatment, iii) HA group as control group but rats treated with HA, iv) ADMSCs group as control but rats treated with ADMSCs v) HA + ADMSCs group as ADMSCs but rats treated with ADMSCs preconditioned with CD44-tageted HA for 14 days. We found that treattment with either ADMSCs or HA + ADMSCs caused significant decrease in the elevated serum creatinine and BUN and malondialdehyde (MDA) concentrations and expression of TGF-β1, fibronectin, collagen type I, inducible nitric oxide synthease (iNOS) and microRNAs (miR-21, miR-17-5p, miR-10a) in kidney and significant increase in creatinine clearance, superoxide dismutase (SOD), reduced glutathione (GSH) and the expression of Bcl2, vascular endothelial growth factor (VEGF), Wnt/β-catenin pathway genes in kidney compared to control group (p