Short-Chain Poly(γ-diaminobutanoic acid), A Poly(amino acid) Produced by a Marine Bacteria Bacillus pumilus

One marine bacteria Bacillus pumilus was isolated using allura red as ε-poly-L-lysine (ε-PL) secretion indicator. But actually the product was identified as poly-γ-L-diaminobutanoic acid (γ-PAB) by ionization-time-of-flight mass spectrometry, not coproduced with ε-PL. The polymerization degree of γ-...

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Bibliographic Details
Published in:Current microbiology 2021-04, Vol.78 (4), p.1142-1149
Main Authors: Li, Shu, Yao, Yao, Hu, Shengjie, Chen, Junru
Format: Article
Language:English
Subjects:
Amino Acids
Bacillus pumilus
Bacteria
Biomedical and Life Sciences
Bioreactors
Biotechnology
Fermentation
Ionization
Ions
Life Sciences
Lysine
Mass spectrometry
Mass spectroscopy
Microbiology
pH control
pH effects
Poly-L-lysine
Polylysine
Streptomyces
Yeasts
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Summary:One marine bacteria Bacillus pumilus was isolated using allura red as ε-poly-L-lysine (ε-PL) secretion indicator. But actually the product was identified as poly-γ-L-diaminobutanoic acid (γ-PAB) by ionization-time-of-flight mass spectrometry, not coproduced with ε-PL. The polymerization degree of γ-PAB was 4–22, namely short-chain γ-PAB, compared with that in S. celluloflavus , and it exhibited stronger inhibitory activities against yeasts than long-chain γ-PAB but weaker activities against bacteria. The fermentative behavior of B. pumilus was investigated, and the γ-PAB production was 38.6 mg/L in shake flask and was enhanced to 284.2 mg/L in 5-L bioreactor by a pH control strategy. Interestingly, the suitable pH for B. pumilus to produce γ-PAB was 4.8, different from 4.0 for current Streptomyces strains, which suggests a potential new metabolic mechanism in B. pumilus as a novel γ-PAB producer. No studies on short-chain γ-PAB production in bacteria have been reported previously and we considered that this is a new discovery in the field of homopolymer research.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-021-02371-6