Variations in the mRNA expression level of UDP-GlcNAc epimerase/ManNAc kinase and neuraminidase 1 genes in organs of type 2 diabetic animals

Sialic acid and its associated metabolic enzymes have emerged as important components of the pathophysiology of type 2 diabetes (T2D). There is an elevation in the serum concentration of sialic acid in humans and animals with T2D. The present study investigated the modulation of mRNA expression leve...

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Bibliographic Details
Published in:Glycoconjugate journal 2021-02, Vol.38 (1), p.129-134
Main Authors: Inim, Mayen David, Ibrahim, Mohammed Auwal, Isah, Murtala Bindawa, Onyike, Elewechi
Format: Article
Language:English
Subjects:
Acids
Adipose tissue
Animals
Biochemistry
Biomedical and Life Sciences
Brain - enzymology
Colon
Colon - enzymology
Diabetes
Diabetes mellitus (non-insulin dependent)
Diabetes Mellitus, Experimental - enzymology
Diabetes Mellitus, Experimental - genetics
Diabetes Mellitus, Type 2 - enzymology
Diabetes Mellitus, Type 2 - genetics
Epimerase
Exo-a-sialidase
Gene expression
Gene Expression Regulation, Enzymologic
Genes
Kidneys
Life Sciences
Liver
Liver - enzymology
Multienzyme Complexes - genetics
Musculoskeletal system
N-Acetylglucosamine
N-Acetylneuraminic Acid - blood
N-Acetylneuraminic Acid - metabolism
Neuraminidase - genetics
Pancreas
Pancreas - enzymology
Pathology
Rats
Rats, Wistar
Real-Time Polymerase Chain Reaction
Rodents
Short Communication
Skeletal muscle
Spleen
Spleen - enzymology
Streptozocin
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Summary:Sialic acid and its associated metabolic enzymes have emerged as important components of the pathophysiology of type 2 diabetes (T2D). There is an elevation in the serum concentration of sialic acid in humans and animals with T2D. The present study investigated the modulation of mRNA expression level of UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase ( GNE ) and neuraminidase 1 ( NEU1 ) genes in some organs of type 2 diabetic rats. T2D was induced using fructose-streptozotocin model and eight weeks after the induction of diabetes, sialic acid was assayed in the blood and organs (adipose tissue, brain, colon, kidney, liver, pancreas, skeletal muscle and spleen) followed by quantification of mRNA expression level of GNE and NEU1 genes by qPCR. The results showed a significant (P 
ISSN:0282-0080
1573-4986
DOI:10.1007/s10719-021-09979-7