Short-chain fatty acid butyrate induces IL-10-producing B cells by regulating circadian-clock-related genes to ameliorate Sjögren's syndrome

• Published in: Journal of autoimmunity 2021-05, Vol.119, p.102611-102611, Article 102611
• Main Authors: Kim, Da Som, Woo, Jin Seok, Min, Hong-Ki, Choi, Jeong-Won, Moon, Jeong Hyeon, Park, Min-Jung, Kwok, Seung-Ki, Park, Sung-Hwan, Cho, Mi-La
• Format: Article
• Language: English
• Subjects: Animals; B cells; B-Lymphocytes - immunology; B-Lymphocytes - metabolism; Basic-Leucine Zipper Transcription Factors - metabolism; Biomarkers; Butyrates - metabolism; Butyrates - pharmacology; Cell Differentiation - drug effects; Cell Differentiation - genetics; Circadian Clocks - genetics; Disease Models, Animal; Disease Susceptibility; Gene Expression Regulation; Immunohistochemistry; Immunophenotyping; Interleukin-10 - biosynthesis; Interleukin-17 - metabolism; Mice; Mice, Inbred NOD; Mice, Knockout; Models, Biological; Nuclear Receptor Subfamily 1, Group D, Member 1 - metabolism; Nuclear Receptor Subfamily 1, Group F, Member 1 - genetics; Nuclear Receptor Subfamily 1, Group F, Member 1 - metabolism; Salivary Glands - immunology; Salivary Glands - metabolism; Salivary Glands - pathology; Sjogren's Syndrome - etiology; Sjogren's Syndrome - metabolism; Sjogren's Syndrome - pathology; Sjögren's syndrome; Sodium butyrate; Stem Cells - cytology; Stem Cells - drug effects; Stem Cells - metabolism
• ISSN: 0896-8411; 1095-9157
• DOI: 10.1016/j.jaut.2021.102611

Sjögren's syndrome (SS) is an autoimmune disease caused by inflammation of the exocrine gland. The pathological hallmark of SS is the infiltration of lymphocytes into the salivary glands. Increased infiltration of T and B cells into salivary glands exacerbates symptoms of SS. Several recent studies have identified the role of gut microbiota in SS. Butyrate, one of the metabolites of the gut microbiota, regulates T cells; however, its effects on B cells and SS remain unknown. This study determined the therapeutic effect of butyrate on regulating B cells in SS. Various concentrations of butyrate were intraperitoneally injected three times per week in NOD/ShiLtJ (NOD) mice, the prototype animal model for SS, and observed for more than 10 weeks. Whole salivary flow rate and the histopathology of salivary glands were investigated. Human submandibular gland (HSG) cells and B cells in mouse spleen were used to confirm the anti-inflammatory and immunomodulatory effects of butyrate. Butyrate increased salivary flow rate in NOD mice and reduced inflammation of salivary gland tissues. It also regulated cell death and the expression of circadian-clock-related genes in HSG cells. Butyrate induced B cell regulation by increasing IL-10-producing B (B10) cells and decreasing IL-17-producing B cells, through the circadian clock genes RAR-related orphan receptor alpha and nuclear receptor subfamily 1 group D member 1. The findings of this study imply that butyrate may ameliorate SS via reciprocal regulation of IL-10- and IL-17-producing B cells. •Butyrate-producing bacteria are reduced in SS.•Butyrate increases B10 by increasing RORα.•Butyrate decreases IL-17-producing B cells by suppressing NR1D1 and increasing NFIL3.•Butyrate regulates cell death and circadian clock-related genes, BMAL1 and CRY1, in HSG cells.•Butyrate have therapeutic efficacy in SS.