Effect of short-term high temperature on the accumulation of glucosinolates in Brassica rapa

• Published in: Plant physiology and biochemistry 2021-04, Vol.161, p.222-233
• Main Authors: Rao, Shuai-Qi, Chen, Xiao-Qi, Wang, Kuan-Hong, Zhu, Zhu-Jun, Yang, Jing, Zhu, Biao
• Format: Article
• Language: English
• Subjects: Brassica rapa - genetics; Glucosinolates; Glutathione; Pakchoi; Seedlings; Short-term high temperature; Stress; Sulfur; Temperature; Transcription Factors
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2021.02.013

Glucosinolates, an important class of secondary metabolites in cruciferous vegetables, play a crucial role in protecting plants from stress-related damage. The mechanism of glucosinolate synthesis under short-term high temperature stress has not been sufficiently studied. In this work, we investigated the changes in transcription factors, synthetic genes, and related metabolites involved in glucosinolate synthesis by pakchoi seedlings under short-term high temperature stress (40 °C for 8 h). Short-term high temperature stress inhibited the primary sulfur assimilation and the contents of methionine, cysteine and glutathione. The contents of aliphatic and indolic glucosinolates were increased by short-term high temperature stress, whereas the content of 4-methoxy-glucobrassicin increased significantly. During the stress period, the transcript level of glucosinolate related MYB transcription factors had been basically significantly up-regulated, whereas the transcript level of aliphatic and indolic glucosinolate synthetic genes were predominantly up-regulated and down-regulated respectively. In the early recovery period, primary sulfur assimilation up-regulated rapidly, and decreased during the late recovery process. The glucosinolate content and synthesis gene expression act similar to the primary sulfur assimilation, a short up-regulated in early recovery, then all go down at 40 and 48 h after short-term high temperature treatment. •Short-term high temperature stress can increase the content of aliphatic and indolic glucosinolates.•4-methoxy-glucobrassicin has a more rapid response to short-term high temperature stress.•Aliphatic glucosinolates and indolic glucosinolates have different responses to short-term high temperature stress.