New insight into G-quadruplexes; diagnosis application in cancer

Biochemical properties and flexibility of nitrogenous bases allow DNA to fold into higher-order structures. Among different DNA secondary structure, G-quadruplexes (tetrapelexes-G4) - which are formed in guanine rich sequences – have gained more attention because of their biological significance, th...

Full description

Saved in:
Bibliographic Details
Published in:Analytical biochemistry 2021-05, Vol.620, p.114149-114149, Article 114149
Main Authors: Shahsavar, Kosar, Hosseini, Morteza, Shokri, Ehsan, Xu, Guobao
Format: Article
Language:English
Subjects:
Cancer diagnosis
DNA aptamer
Electrochemical
G-quadruplexes
Optical sensor
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Biochemical properties and flexibility of nitrogenous bases allow DNA to fold into higher-order structures. Among different DNA secondary structure, G-quadruplexes (tetrapelexes-G4) - which are formed in guanine rich sequences – have gained more attention because of their biological significance, therapeutic intervention, and application in molecular device and biosensor. G4-quadruplex studies categorize into three main fields, in vivo, in vitro, and in silico. The in vitro field includes G4 synthetic oligonucleotides. This review focuses on the G-quadruplex synthetic aptamers structure features and considers the applicability of G4-aptamers for cancer biomarkers detection. Various biosensing methods will be reviewed based on G-quadruplex aptamers for cancer detection. [Display omitted] •Detection of cancer, as one of the top death causes worldwide, has great importance.•G-quadruplex aptamers are noncanonical DNA structures containing runs of three or four adjacent guanines.•G-quadruplexes serve as a host for multiple ligands that makes them excellent candidates in diagnostic applications.•An updated overview of G-quadruplex based sensors (optical, electrochemical) for cancer diagnosis will be summarized here.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2021.114149