Genome-wide investigation and expression analysis of MACPF gene family reveals its immune role in response to bacterial challenge of Manila clam

In this study, 18 MACPF genes (RpMACPF) were identified and classed into three types (Macrophage-expressed gene 1, Apextrin, and MACPF domain contain protein) based on gene structure and phylogenetic relationship in R. philippinarum. The length of RpMACPF proteins varied from 287 to 785 amino acids....

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2021-05, Vol.113 (3), p.1136-1145
Main Authors: Jiang, Kunyin, Yin, Zhihui, Zhang, Yanming, Xu, Qiaoyue, Yu, Yongchao, Cong, Wanlin, Yan, Xiwu, Nie, Hongtao
Format: Article
Language:English
Subjects:
Animals
Bacteria - genetics
Bivalvia - genetics
Gene family
MACPF
Phylogeny
Response to invading pathogens
RNA-Seq
Ruditapes philippinarum
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Summary:In this study, 18 MACPF genes (RpMACPF) were identified and classed into three types (Macrophage-expressed gene 1, Apextrin, and MACPF domain contain protein) based on gene structure and phylogenetic relationship in R. philippinarum. The length of RpMACPF proteins varied from 287 to 785 amino acids. The molecular weights and Theoretical PI values ranged from 3.2 kDa to 8.7 kDa and 4.7 to 8.6, respectively. RNA-seq data analysis revealed that 14 of 18 RpMACPF genes were highly expressed at the pediveliger larvae stage indicate RpMACPF might contribute to the early development and metamorphosis processes of the R. philippinarum. Besides, we found RpMACPF genes were significantly regulated by pathogen-associated molecular patterns (PAMPs) and Vibrio parahemolyticus, which indicates RpMACPF genes may play significant roles in response to invading pathogens. The results obtained in this work will provide valuable insight into the immune function of MACPF gene in R. philippinarum. •A total of 18 members of MACPF gene family were identified from R. philippinarum.•RpMACPF proteins were clustered into three types, Mpeg1, Ape, and Macpf.•RpMACPF genes may contribute to the early development and metamorphosis processes of the R. philippinarum.•RpMACPF genes might play important roles in response to invading pathogens in R. philippinarum.
ISSN:0888-7543
1089-8646
DOI:10.1016/j.ygeno.2021.02.013