Divergence times and plastid phylogenomics within the intron‐rich order Erythropeltales (Compsopogonophyceae, Rhodophyta)

• Published in: Journal of phycology 2021-06, Vol.57 (3), p.1035-1044
• Main Authors: Preuss, Maren, Verbruggen, Heroen, West, John A., Zuccarello, Giuseppe C., De Clerck, O.
• Format: Article
• Language: English
• Subjects: Algae; ancestral state construction; chloroplast; comparative analysis of introns; Completeness; deep phylogeny; Divergence; diversification; Evolution; Gene order; Genera; Genomes; Glaciation; group II introns; Ice ages; Introns; molecular clock; Morphology; Phylogenetics; Phylogeny; plastid genome; Plastids; red algae; Robustness; Sampling; Sequences; Taxa
• ISSN: 0022-3646; 1529-8817
• DOI: 10.1111/jpy.13159

The advent of high‐throughput sequencing (HTS) has allowed for the use of large numbers of coding regions to produce robust phylogenies. These phylogenies have been used to highlight relationships at ancient diversifications (subphyla, class) and highlight the evolution of plastid genome structure. The Erythropeltales are an order in the Compsopogonophyceae, a group with unusual plastid genomes but with low taxon sampling. We use HTS to produce near complete plastid genomes of all genera, and multiple species within some genera, to produce robust phylogenies to investigate character evolution, dating of divergence in the group, and plastid organization, including intron patterns. Our results produce a fully supported phylogeny of the genera in the Erythropeltales and suggest that morphologies (upright versus crustose) have evolved multiple times. Our dated phylogeny also indicates that the order is very old (~800 Ma), with diversification occurring after the ice ages of the Cryogenian period (750–635 Ma). Plastid gene order is congruent with phylogenetic relationships and suggests that genome architecture does not change often. Our data also highlight the abundance of introns in the plastid genomes of this order. We also produce a nearly complete plastid genome of Tsunamia transpacifica (Stylonematophyceae) to add to the taxon sampling of genomes of this class. The use of plastid genomes clearly produces robust phylogenetic relationships that can be used to infer evolutionary events, and increased taxon sampling, especially in less well‐known red algal groups, will provide additional insights into their evolution.