Neural EGFL like 2 expressed in myoepithelial cells and suppressed breast cancer cell migration

Breast tissue has a branching structure that contains double‐layered cells, consisting primarily of luminal epithelial cells inside and myoepithelial cells outside. Ductal carcinoma in situ (DCIS) still has myoepithelial cells surrounding the cancer cells. However, myoepithelial cells disappear in i...

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Bibliographic Details
Published in:Pathology international 2021-05, Vol.71 (5), p.326-336
Main Authors: Nakamura, Ritsuko, Oyama, Takeru, Inokuchi, Masafumi, Ishikawa, Satoko, Hirata, Miki, Kawashima, Hiroko, Ikeda, Hiroko, Dobashi, Yoh, Ooi, Akishi
Format: Article
Language:English
Subjects:
Adhesion
breast
Breast cancer
Cell adhesion
Cell adhesion & migration
Cell migration
Epithelial cells
Epithelium
Invasiveness
Lesions
luminal epithelial cell
myoepithelial cell
NELL2
Receptors
Robo protein
ROBO3
Tumor cell lines
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Summary:Breast tissue has a branching structure that contains double‐layered cells, consisting primarily of luminal epithelial cells inside and myoepithelial cells outside. Ductal carcinoma in situ (DCIS) still has myoepithelial cells surrounding the cancer cells. However, myoepithelial cells disappear in invasive ductal carcinoma. In this study, we detected expression of neural EGFL like (NELL) 2 and one of its receptors, roundabout guidance receptor (ROBO) 3, in myoepithelial and luminal epithelial cells (respectively) in normal breast tissue. NELL2 also was expressed in myoepithelial cells surrounding the non‐cancerous intraductal proliferative lesions and DCIS. However, the expression level and proportion of NELL2‐positive cells in DCIS were lower than those in normal and non‐cancerous intraductal proliferative lesions. ROBO3 expression was decreased in invasive ductal carcinoma compared to that in normal and non‐cancerous intraductal proliferative lesions. An evaluation of NELL2's function in breast cancer cell lines demonstrated that full‐length NELL2 suppressed cell adhesion and migration in vitro. In contrast, the N‐terminal domain of NELL2 increased cell adhesion in the early phase and migration in vitro in some breast cancer cells. These results suggested that full‐length NELL2 protein, when expressed in myoepithelial cells, might serve as an inhibitor of breast cancer cell migration.
ISSN:1320-5463
1440-1827
DOI:10.1111/pin.13087