Glaciibacter flavus sp. nov., isolated from a lichen sample

• Published in: Archives of microbiology 2021-07, Vol.203 (5), p.2439-2444
• Main Authors: An, De-Feng, Jiang, Long-Qian, Zhang, Kun, Li, Gui-Ding, Wang, Xin-Yu, Jiang, Ming-Guo, Lang, Lei, Wang, Li-Song, Imhoff, Johannes F., Jiang, Cheng-Lin, Jiang, Yi
• Format: Article
• Language: English
• Subjects: Actinobacteria; Amino acids; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cardiolipin; Catalase; Cell Biology; Deoxyribonucleic acid; DNA; Ecology; Fatty acids; Flagella; Galactose; Gene sequencing; Glaciibacter; Glycolipids; Gram-positive bacteria; Hybridization; Lichens; Life Sciences; Lipids; Menaquinones; Microbial Ecology; Microbiology; New species; Nucleotide sequence; Nucleotides; Original Paper; Peptidoglycans; pH effects; Phosphatidylglycerol; Phylogenetics; Phylogeny; Rhamnose; Ribose; rRNA 16S; Sodium chloride; Sugar
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-021-02247-0

A novel Actinobacterium strain YIM 131861  T , was isolated from lichen collected from the South Bank Forest of the Baltic Sea, Germany. It was Gram-stain-positive, strictly aerobic, catalase positive and oxidase negative, yellow pigmented. Cells were motile with a polar flagellum, irregular rod shaped and did not display spore formation. The strain grew at 15 − 30 °C (optimum 25 °C), at pH 6.0 − 10.0 (optimum pH 7.0) and in the presence of 0 − 1.5% (w/v) NaCl (optimum 1%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 131861  T belonged to the genus Glaciibacter , and exhibited a high sequence similarity (96.4%) with Glaciibacter superstes NBRC 104264  T . The genomic DNA G + C content of strain YIM 131861  T was 68.2 mol%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain YIM 131861  T and Glaciibacter superstes NBRC 104264  T were 73.2 and 19.9% based on the draft genome sequence. The cell-wall peptidoglycan type was B2 γ and contained the 2, 4-diaminobutyric acid as the diagnostic amino acid. Whole cell sugars were galactose, rhamnose, ribose and glucose. It contained MK-12 and MK-13 as the predominant menaquinones. The major cellular fatty acids (> 10%) were identified as anteiso -C 15:0 , iso -C 16:0 and anteiso -C 17:0 . The polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain YIM 131861  T should belong to the genus Glaciibacter and represents a novel species of the genus Glaciibacter , for which the name Glaciibacter flavus sp. nov. is proposed. The type strain is YIM 131861  T (= CGMCC 1.16588  T  = NBRC 113572  T ).