Regulation and function of leptin during ovarian follicular development in cows

• Published in: Animal reproduction science 2021-04, Vol.227, p.106689-106689, Article 106689
• Main Authors: Martins, Kauê R., Haas, Cristina S., Rovani, Monique T., Moreira, Fabiana, Goetten, André L.F., Ferst, Juliana G., Portela, Valério. M., Duggavathi, Raj, Bordignon, Vilceu, Gonçalves, Paulo B.D., Gasperin, Bernardo G., Lucia, Thomaz
• Format: Article
• Language: English
• Subjects: Cattle; Follicular growth; Granulosa cells; Leptin; Steroidogenesis
• ISSN: 0378-4320; 1873-2232
• DOI: 10.1016/j.anireprosci.2021.106689

•Leptin and leptin receptor were detected in granulosa and theca cells of follicles.•LEPROT, ADIPOR1 and ADIPOR2 mRNA was lower in subordinate follicles.•Intrafollicular injection of leptin did not affect dominant follicle growth.•Leptin did not regulate steroidogenic and apoptotic genes mRNA in granulosa cells. Although it is well documented that leptin signals the body nutritional status to the brain, mechanisms of leptin regulation at the ovary are not well understood. This study was conducted to determine whether there was leptin and the receptor for leptin (LEPR) in cattle ovarian follicles and to investigate potential actions of leptin on follicular growth in vivo and on regulation of granulosa cell functions in vitro. There was leptin and LEPR in granulosa and theca cells of dominant and subordinate follicles, with greater immunostaining for leptin in granulosa cells of subordinate follicles. There was a lesser relative abundance of leptin receptor gene-related protein (LEPROT) and of the adiponectin receptors 1 (ADIPOR1) and 2 (ADIPOR2) mRNA transcripts in granulosa cells of subordinate than dominant follicles (P < 0.05). Intrafollicular injection of either 100 or 1000 ng/mL leptin did not affect the diameter and the growth of dominant follicles (P> 0.05). Supplementation of in vitro culture medium with different leptin concentations did not affect (P > 0.05) the relative abundance of hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (HSD3B1), cytochrome P450 family 11 subfamily A member 1 (CYP11A1), signal transducer and activator of transcription 3 (STAT3) and X-linked inhibitor of apoptosis protein (XIAP) mRNA transcripts in granulosa cells. These findings indicate that leptin and LEPR are present in the follicular cells of cattle ovaries, but leptin apparently does not have essential functions in steroidogenesis and growth of dominant follicles.