An ultrasensitive heart-failure BNP biosensor using B/N co-doped graphene oxide gel FET

• Published in: Biosensors & bioelectronics 2021-05, Vol.180, p.113114-113114, Article 113114
• Main Authors: Novodchuk, I., Kayaharman, M., Ausri, I.R., Karimi, R., Tang, X.S., Goldthorpe, I.A., Abdel-Rahman, E., Sanderson, J., Bajcsy, M., Yavuz, M.
• Format: Article
• Language: English
• Subjects: Biosensing Techniques; Biosensor; FET; Graphene-based; Graphite; Heart Failure; Humans; Transistors, Electronic
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2021.113114

Heart failure (HF) is the number one cause of death in the world. B-type natriuretic peptide (BNP) is a recognized biomarker for HF and can be used for early detection. Field effect transistor (FET) biosensors have the ability to sense BNP in much shorter times than conventional clinical studies. The lowest limit of detection (LOD) of state-of-the-art HF FET biosensors is 100 fM and detection ranges are short, being less than 4 orders of magnitude. In this work, a B/N co-doped graphene oxide (GO) gel (BN-GO) was used as the channel material in an FET biosensor targeting BNP. The sensor was able to sense BNP in as little as 2 min, with an LOD as low as 10 aM and a wide linear detection range of 10 aM–1 μM, stretching over 11 orders of magnitude. The biosensor showed great selectivity and minimal response towards K+ and OH− ions and the human epidermal growth factor receptor (HER2) protein. This biosensor serves as a proof-of-concept of the ability of BN-GO gel FETs to be used for ultrasensitive biosensors. •Fabrication of antibody-functionalized BN-GO gel FET biosensor for early detection of heart failure.•The biosensor is ultrasensitive with a limit of detection as low as 10 aM.•A wide dynamic range of 10 aM-1 μM is achieved.•The biosensor shows a short detection time of 2 min, shorter than most state-of-the-art FET BNP biosensors.