Combination of entomopathogenic nematodes with acaricides or essential oil of Lippia triplinervis against Rhipicephalus microplus (Acari: Ixodidae)

• Published in: Veterinary parasitology (Amsterdam) 2021-01, Vol.23, p.100526-100526, Article 100526
• Main Authors: Monteiro, Caio, Lage, Tiago Coelho de Assis, Marchesini, Paula, Vale, Letícia, Perinotto, Wendell Marcelo de Souza, Lopes, Welber Daniel Zanetti, Fernandes, Sergio Antônio, Bittencourt, Vânia Rita Elias Pinheiro, Furlong, John, Prata, Márcia Cristina de Azevedo
• Format: Article
• Language: English
• Subjects: Carvacrol; Formamidine; Organophosphate; Pyrethroid; Southern cattle tick; Thymol
• ISSN: 2405-9390; 2405-9390
• DOI: 10.1016/j.vprsr.2020.100526

This study aimed to evaluate the combination effect of Heterorhabditis bacteriophora HP88 and H. indica LPP1, with the acaricides deltamethrin, amitraz and chlorfenvinphos, and the essential oil (EO) of Lippia triplinervis, against engorged females of Rhipicephalus microplus. In order to verify the effect of acaricides and EO, the adult immersion test was used, and in the groups treated only with entomopathogenic nematodes (EPNs), 150 infective juveniles were used per female. In the treatments with nematodes in combination with the acaricides or EO, the females were immersed in the solutions (acaricide or EO) and then transferred to Petri dishes for application of the nematodes. The treatment with acaricides resulted in a control percentage lower than 70%, except in the group treated with chlorfenvinphos in the second experiment (84.3%). The control percentage was 73% for L. triplinervis EO, and greater than 90% in all the groups treated with nematodes. For treatments with EPNs combined with the acaricides or EO, the efficacy was greater than 95% (except for deltamethrin + HP88), and reached 100% in the treatment with LPP1 + amitraz. It can be concluded that the EPNs at the concentrations tested were compatible with the acaricides deltamethrin, amitraz and chlorfenvinphos, and with the EO of L. triplinervis. These combinations enhance the effect of these control agents. [Display omitted] •Deltamethrin, amitraz and chlorfenvinphos showed low efficacy on cattle tick.•The essential oil of Lippia triplinervis (EO) presented efficacy of 73.3%.•The entomopathogenic nematodes (NEPs), HP88 and LPP1 presented efficacy near to 90%.•Combination of EPNs with acaricides or EO presented efficacy near to 100%.•Combination of Heterorhabitidis indica LPP1 + amitraz presented efficacy to 100%.