Pericardial fluid and vascular tissue engineering: A preliminary study

BACKGROUND: The heart is surrounded by a membrane called pericardium or pericardial cavity. OBJECTIVE: In this study, we investigated the pericardial fluid (PF) for coating polycaprolactone (PCL) scaffolds. PFS, which is a PF component, was used for the coating material. In addition to using PFS for...

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Bibliographic Details
Published in:Bio-medical materials and engineering 2021-01, Vol.32 (2), p.101-113
Main Authors: Sönmezer, Dilek, Lati̇foğlu, Fatma, Toprak, Güler, Düzler, Ayhan, İşoğlu, İsmail Alper
Format: Article
Language:English
Subjects:
Assaying
Biocompatibility
Biomedical materials
Cell viability
Coating
Coatings
Fetal calf serum
Microscopy
Pericardium
Polycaprolactone
Protective coatings
Scaffolds
Scanning electron microscopy
Tissue engineering
Vascular tissue
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Summary:BACKGROUND: The heart is surrounded by a membrane called pericardium or pericardial cavity. OBJECTIVE: In this study, we investigated the pericardial fluid (PF) for coating polycaprolactone (PCL) scaffolds. PFS, which is a PF component, was used for the coating material. In addition to using PFS for surface coating, MED and fetal bovine serum (FBS) were also used for comparison. METHODS: Pericardial fluid cells (PFSc) isolated from PF were cultured on coated PCL scaffolds for 1, 3, and 5 days. Cell viability was determined using 3-(4, 5-di-methylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. RESULTS: The MTT assay results showed that the viability of cells on PCL scaffold coated with PFS increased over time (P < 0.005), and cell viability was significantly different between PCL scaffolds coated with PFS and non-coated PCL scaffolds. However, cell viability was significantly higher in the PCL scaffolds coated with PFS than non-coated and coated with FBS, MED, and PCL scaffolds. Scanning electron microscopy (SEM) microscopy images and MTT assay indicated that PFSc are attached, proliferated, and spread on PCL scaffolds, especially on PCL scaffolds coated with PFS. CONCLUSIONS: These results suggest that PFS is a biocompatible material for surface modification of PCL scaffolds, which can be used as a suitable material for tissue engineering applications.
ISSN:0959-2989
1878-3619
DOI:10.3233/BME-196014