Reversible Control of DNA Binding with Cucurbit[8]uril-Induced Supramolecular 4,4′-Bipyridinium–Peptide Dimers

Many cellular processes in living organisms are regulated by complex regulatory networks, built from noncovalent interactions between relatively few proteins that perform their functions by switching between homo- and heterooligomeric assemblies or mono- and bivalent states. Herein, we demonstrate t...

Full description

Saved in:
Bibliographic Details
Published in:Bioconjugate chemistry 2021-03, Vol.32 (3), p.507-511
Main Authors: Novo, Paula, García, Marcos D, Peinador, Carlos, Pazos, Elena
Format: Article
Language:English
Subjects:
Binding
Cellular communication
Conjugation
Deoxyribonucleic acid
Dimerization
Dimers
DNA
External stimuli
Target recognition
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Many cellular processes in living organisms are regulated by complex regulatory networks, built from noncovalent interactions between relatively few proteins that perform their functions by switching between homo- and heterooligomeric assemblies or mono- and bivalent states. Herein, we demonstrate that the conjugation of a 4,4′-bipyridinium scaffold to the basic region of the GCN4 bZip transcription factor can be exploited to control the dimerization of the conjugate by formation of a supramolecular complex with cucurbit[8]­uril. Importantly, this supramolecular complex is able to specifically recognize its target dsDNA, and this binding can be reversibly switched by the application of external stimuli.
ISSN:1043-1802
1520-4812
DOI:10.1021/acs.bioconjchem.1c00063