Reversible Control of DNA Binding with Cucurbit[8]uril-Induced Supramolecular 4,4′-Bipyridinium–Peptide Dimers

Many cellular processes in living organisms are regulated by complex regulatory networks, built from noncovalent interactions between relatively few proteins that perform their functions by switching between homo- and heterooligomeric assemblies or mono- and bivalent states. Herein, we demonstrate t...

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Published in:Bioconjugate chemistry 2021-03, Vol.32 (3), p.507-511
Main Authors: Novo, Paula, García, Marcos D, Peinador, Carlos, Pazos, Elena
Format: Article
Language:English
Subjects:
Binding
Cellular communication
Conjugation
Deoxyribonucleic acid
Dimerization
Dimers
DNA
External stimuli
Target recognition
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cited_by cdi_FETCH-LOGICAL-a434t-4ffdcb8db8aa6158ead203cfbac71deec1624e4e0417a66a224681e70c49ca113
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container_title Bioconjugate chemistry
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description Many cellular processes in living organisms are regulated by complex regulatory networks, built from noncovalent interactions between relatively few proteins that perform their functions by switching between homo- and heterooligomeric assemblies or mono- and bivalent states. Herein, we demonstrate that the conjugation of a 4,4′-bipyridinium scaffold to the basic region of the GCN4 bZip transcription factor can be exploited to control the dimerization of the conjugate by formation of a supramolecular complex with cucurbit[8]­uril. Importantly, this supramolecular complex is able to specifically recognize its target dsDNA, and this binding can be reversibly switched by the application of external stimuli.
doi_str_mv 10.1021/acs.bioconjchem.1c00063
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source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects Binding
Cellular communication
Conjugation
Deoxyribonucleic acid
Dimerization
Dimers
DNA
External stimuli
Target recognition
title Reversible Control of DNA Binding with Cucurbit[8]uril-Induced Supramolecular 4,4′-Bipyridinium–Peptide Dimers
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