Gga-miR-181a modulates ANP32A expression and inhibits MDCC-MSB-1 cell

Marek’s disease (MD), a highly contagious T cell lymphoid neoplasia disease of chickens, causes huge economic losses to the poultry industry. It is the only one tumor disease which can be prevented by vaccine in chickens; therefore, MD is considered to be an excellent model to study the pathogenesis...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 2021-03, Vol.57 (3), p.272-279
Main Authors: Li, X., Zhao, C., Han, B., Qu, L., Liu, C., Yang, N., Lian, L.
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Assaying
Biomedical and Life Sciences
Cell adhesion & migration
Cell Biology
Cell Culture
Cell migration
Chickens
Developmental Biology
Economic impact
Life Sciences
Lymphocytes
Lymphocytes T
Lymphoma
Marek's disease
mRNA
Pathogenesis
Regulators
Reporter gene
Stem Cells
Transfection
Tumorigenesis
Vaccines
Viruses
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Summary:Marek’s disease (MD), a highly contagious T cell lymphoid neoplasia disease of chickens, causes huge economic losses to the poultry industry. It is the only one tumor disease which can be prevented by vaccine in chickens; therefore, MD is considered to be an excellent model to study the pathogenesis of virus-induced cancer. Recently, abundant evidences have verified that miRNAs are regulators in the process of neoplastic transformation. In our previous study on miRNome analysis of MDV-induced lymphoma in chicken, we found that gga-miR-181a was downregulated drastically in MDV-infected spleens. To further investigate the role of gga-miR-181a in MDV-induced lymphomagenesis, we performed cell migration assay, and the results suggested that gga-miR-181a suppressed the migration of MDV-transformed lymphoid cell (MSB-1). Subsequently, luciferase reporter gene assay revealed that acidic nuclear phosphoprotein 32A ( ANP32A ) was a functional target gene of gga-miR181a. Real-time PCR and western blot assay showed that the mRNA and protein levels of ANP32A were downregulated in gga-miR-181a mimic group at 48-h and 96-h post-transfection, respectively, indicating that ANP32A was modulated by gga-miR-181a. All the results suggested that gga-miR-181a was an inhibitor in MSB-1 cell migration. ANP32A was a direct target gene of gga-miR-181a and they were implicated in MD lymphoma tumorigenesis.
ISSN:1071-2690
1543-706X
DOI:10.1007/s11626-021-00550-0