Gga-miR-181a modulates ANP32A expression and inhibits MDCC-MSB-1 cell

• Published in: In vitro cellular & developmental biology. Animal 2021-03, Vol.57 (3), p.272-279
• Main Authors: Li, X., Zhao, C., Han, B., Qu, L., Liu, C., Yang, N., Lian, L.
• Format: Article
• Language: English
• Subjects: Animal Genetics and Genomics; Assaying; Biomedical and Life Sciences; Cell adhesion & migration; Cell Biology; Cell Culture; CELL GROWTH/DIFFERENTIATION/APOPTOSIS; Cell migration; Chickens; Developmental Biology; Economic impact; Life Sciences; Lymphocytes; Lymphocytes T; Lymphoma; Marek's disease; mRNA; Pathogenesis; Regulators; Reporter gene; Stem Cells; Transfection; Tumorigenesis; Vaccines; Viruses
• ISSN: 1071-2690; 1543-706X
• DOI: 10.1007/s11626-021-00550-0

Marek's disease (MD), a highly contagious T cell lymphoid neoplasia disease of chickens, causes huge economic losses to the poultry industry. It is the only one tumor disease which can be prevented by vaccine in chickens; therefore, MD is considered to be an excellent model to study the pathogenesis of virus-induced cancer. Recently, abundant evidences have verified that miRNAs are regulators in the process of neoplastic transformation. In our previous study on miRNome analysis of MDV-induced lymphoma in chicken, we found that gga-miR-181a was downregulated drastically in MDV-infected spleens. To further investigate the role of gga-miR-181a in MDV-induced lymphomagenesis, we performed cell migration assay, and the results suggested that gga-miR-181a suppressed the migration of MDV-transformed lymphoid cell (MSB-1). Subsequently, luciferase reporter gene assay revealed that acidic nuclear phosphoprotein 32A (ANP32A) was a functional target gene of gga-miR181a. Real-time PCR and western blot assay showed that the mRNA and protein levels of ANP32A were downregulated in gga-miR181a mimic group at 48-h and 96-h post-transfection, respectively, indicating that ANP32A was modulated by gga-miR- 181a. All the results suggested that gga-miR-181a was an inhibitor in MSB-1 cell migration. ANP32A was a direct target gene of gga-miR-181a and they were implicated in MD lymphoma tumorigenesis.