Lactococcus-based vaccine against brucellosis: IgG immune response in mice with rOmp16-IL2 fusion protein

This study was designed to introduce the recombinant Lactococcus lactis MG1363 as a cell factory candidate for production of recombinant Brucella melitensis Omp16-Human IL2 (r-Omp16-IL2) and to suggest it as a promising safe, non-pathogenic mucosal live vaccine against brucellosis. Three groups of B...

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Bibliographic Details
Published in:Archives of microbiology 2021-07, Vol.203 (5), p.2591-2596
Main Authors: Rezaei, Marzieh, Rabbani-khorasgani, Mohammad, Zarkesh-Esfahani, Sayyed Hamid, Emamzadeh, Rahman, Abtahi, Hamid
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biotechnology
Brucellosis
Cell Biology
Ecology
Fusion protein
Immune response
Immune system
Immunization
Immunoglobulin G
Interleukin 2
Life Sciences
Microbial Ecology
Microbiology
Mucosa
Original Paper
Outer membrane proteins
Proteins
Vaccines
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Summary:This study was designed to introduce the recombinant Lactococcus lactis MG1363 as a cell factory candidate for production of recombinant Brucella melitensis Omp16-Human IL2 (r-Omp16-IL2) and to suggest it as a promising safe, non-pathogenic mucosal live vaccine against brucellosis. Three groups of BALB/c mice (10 mice per group) were intragastrically administrated with phosphate-buffered saline (PBS), L. lactis harboring the empty pAMJ2008 plasmid and with L. lactis expressing rOmp-IL2. The first two groups were classified as control groups and the third one is indicated as treatment group. Another group was injected by the intraperitoneal (i.p.) route with purified rOmp16-IL2 protein. The total serum IgG of each group was assessed with indirect ELISAs at two days before immunization and also two weeks after the last immunization. Results showed that BALB/c mice intragastrically administrated with L. lactis expressing rOmp-IL2 had dominant IgG response compared to the control (PBS administrated) group ( P  
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-021-02241-6