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Exosomes from adipose-derived mesenchymal stem cells promote survival of fat grafts by regulating macrophage polarization via let-7c
Abstract The rate of fat graft survival is a critical aspect of successful surgery and has been a matter of concern for over 20 years. Owing to their anti-inflammatory effects and regenerative property, adipose-derived mesenchymal stem cells (AD-MSCs) have been adapted for clinical application in fa...
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Published in: | Acta biochimica et biophysica Sinica 2021-04, Vol.53 (4), p.501-510 |
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creator | Hao, Xiaoyan Guo, Yuan Wang, Rui Yu, Xueyuan He, Lin Shu, Maoguo |
description | Abstract
The rate of fat graft survival is a critical aspect of successful surgery and has been a matter of concern for over 20 years. Owing to their anti-inflammatory effects and regenerative property, adipose-derived mesenchymal stem cells (AD-MSCs) have been adapted for clinical application in fat grafting, although the mechanism underlying their action remains unclear. Recently, exosomes derived from MSCs were suggested as a better alternative, and these exosomes have also been applied in diverse clinical therapies. Accumulating evidence suggests that MSCs modulate macrophage differentiation via exosome secretion, and the connection between macrophage regulation and the rate of fat graft survival has been established. Here, we identified that let-7c, the key factor in the regulatory process, is shuttled by AD-MSC-derived exosomes to downregulate the transcription factor CCAAT/enhancer-binding protein (C/EBP)-δ. The downregulation of C/EBP-δ resulted in the attenuation of pro-inflammatory M1 macrophages and elevation of anti-inflammatory M2 macrophages. These results suggest that AD-MSC-derived exosomes promote the survival of fat grafts by regulating macrophage polarization via let-7c. This is the first study to elucidate the mechanism underlying the promotion of the fat graft survival rate by AD-MSCs and to evaluate the immunotherapeutic potential of AD-MSC-derived exosomes in fat grafting. |
doi_str_mv | 10.1093/abbs/gmab018 |
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The rate of fat graft survival is a critical aspect of successful surgery and has been a matter of concern for over 20 years. Owing to their anti-inflammatory effects and regenerative property, adipose-derived mesenchymal stem cells (AD-MSCs) have been adapted for clinical application in fat grafting, although the mechanism underlying their action remains unclear. Recently, exosomes derived from MSCs were suggested as a better alternative, and these exosomes have also been applied in diverse clinical therapies. Accumulating evidence suggests that MSCs modulate macrophage differentiation via exosome secretion, and the connection between macrophage regulation and the rate of fat graft survival has been established. Here, we identified that let-7c, the key factor in the regulatory process, is shuttled by AD-MSC-derived exosomes to downregulate the transcription factor CCAAT/enhancer-binding protein (C/EBP)-δ. The downregulation of C/EBP-δ resulted in the attenuation of pro-inflammatory M1 macrophages and elevation of anti-inflammatory M2 macrophages. These results suggest that AD-MSC-derived exosomes promote the survival of fat grafts by regulating macrophage polarization via let-7c. This is the first study to elucidate the mechanism underlying the promotion of the fat graft survival rate by AD-MSCs and to evaluate the immunotherapeutic potential of AD-MSC-derived exosomes in fat grafting.</description><identifier>ISSN: 1672-9145</identifier><identifier>EISSN: 1745-7270</identifier><identifier>DOI: 10.1093/abbs/gmab018</identifier><identifier>PMID: 33704368</identifier><language>eng</language><publisher>UK: Oxford University Press</publisher><ispartof>Acta biochimica et biophysica Sinica, 2021-04, Vol.53 (4), p.501-510</ispartof><rights>The Author(s) 2021. Published by Oxford University Press on behalf of the Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2021</rights><rights>The Author(s) 2021. Published by Oxford University Press on behalf of the Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c323t-881cbb916b2f8358ab0257329c48611efd251d375970d0e323394696665e63e3</citedby><cites>FETCH-LOGICAL-c323t-881cbb916b2f8358ab0257329c48611efd251d375970d0e323394696665e63e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33704368$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hao, Xiaoyan</creatorcontrib><creatorcontrib>Guo, Yuan</creatorcontrib><creatorcontrib>Wang, Rui</creatorcontrib><creatorcontrib>Yu, Xueyuan</creatorcontrib><creatorcontrib>He, Lin</creatorcontrib><creatorcontrib>Shu, Maoguo</creatorcontrib><title>Exosomes from adipose-derived mesenchymal stem cells promote survival of fat grafts by regulating macrophage polarization via let-7c</title><title>Acta biochimica et biophysica Sinica</title><addtitle>Acta Biochim Biophys Sin (Shanghai)</addtitle><description>Abstract
The rate of fat graft survival is a critical aspect of successful surgery and has been a matter of concern for over 20 years. Owing to their anti-inflammatory effects and regenerative property, adipose-derived mesenchymal stem cells (AD-MSCs) have been adapted for clinical application in fat grafting, although the mechanism underlying their action remains unclear. Recently, exosomes derived from MSCs were suggested as a better alternative, and these exosomes have also been applied in diverse clinical therapies. Accumulating evidence suggests that MSCs modulate macrophage differentiation via exosome secretion, and the connection between macrophage regulation and the rate of fat graft survival has been established. Here, we identified that let-7c, the key factor in the regulatory process, is shuttled by AD-MSC-derived exosomes to downregulate the transcription factor CCAAT/enhancer-binding protein (C/EBP)-δ. The downregulation of C/EBP-δ resulted in the attenuation of pro-inflammatory M1 macrophages and elevation of anti-inflammatory M2 macrophages. These results suggest that AD-MSC-derived exosomes promote the survival of fat grafts by regulating macrophage polarization via let-7c. This is the first study to elucidate the mechanism underlying the promotion of the fat graft survival rate by AD-MSCs and to evaluate the immunotherapeutic potential of AD-MSC-derived exosomes in fat grafting.</description><issn>1672-9145</issn><issn>1745-7270</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kD1PwzAQhi0EoqWwMSNvMBDqj9hORlSVD6kSS_fISS5pUBwHO6koMz8cVy2MTD75nnt19yB0TckDJSmf6zz389ronNDkBE2pikWkmCKnoZaKRSmNxQRdeP9OCJeSknM04VyRmMtkir6Xn9ZbAx5Xzhqsy6a3HqISXLOFEocGdMVmZ3SL_QAGF9C2HveBtQNgP7ptsw09W-FKD7h2uho8znfYQT22emi6GhtdONtvdA24t612zVf4tx3eNhq3MESquERnlW49XB3fGVo_LdeLl2j19vy6eFxFBWd8iJKEFnmeUpmzKuEiCSczoThLiziRlEJVMkFLrkSqSEkgzPA0lqmUUoDkwGfo7hAb1v8YwQ-Zafz-IN2BHX3GRDCkuEhZQO8PaFjdewdV1rvGaLfLKMn22rO99uyoPeA3x-QxN1D-wb-eA3B7AOzY_x_1AwHOjkY</recordid><startdate>20210401</startdate><enddate>20210401</enddate><creator>Hao, Xiaoyan</creator><creator>Guo, Yuan</creator><creator>Wang, Rui</creator><creator>Yu, Xueyuan</creator><creator>He, Lin</creator><creator>Shu, Maoguo</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20210401</creationdate><title>Exosomes from adipose-derived mesenchymal stem cells promote survival of fat grafts by regulating macrophage polarization via let-7c</title><author>Hao, Xiaoyan ; Guo, Yuan ; Wang, Rui ; Yu, Xueyuan ; He, Lin ; Shu, Maoguo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c323t-881cbb916b2f8358ab0257329c48611efd251d375970d0e323394696665e63e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hao, Xiaoyan</creatorcontrib><creatorcontrib>Guo, Yuan</creatorcontrib><creatorcontrib>Wang, Rui</creatorcontrib><creatorcontrib>Yu, Xueyuan</creatorcontrib><creatorcontrib>He, Lin</creatorcontrib><creatorcontrib>Shu, Maoguo</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta biochimica et biophysica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hao, Xiaoyan</au><au>Guo, Yuan</au><au>Wang, Rui</au><au>Yu, Xueyuan</au><au>He, Lin</au><au>Shu, Maoguo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exosomes from adipose-derived mesenchymal stem cells promote survival of fat grafts by regulating macrophage polarization via let-7c</atitle><jtitle>Acta biochimica et biophysica Sinica</jtitle><addtitle>Acta Biochim Biophys Sin (Shanghai)</addtitle><date>2021-04-01</date><risdate>2021</risdate><volume>53</volume><issue>4</issue><spage>501</spage><epage>510</epage><pages>501-510</pages><issn>1672-9145</issn><eissn>1745-7270</eissn><abstract>Abstract
The rate of fat graft survival is a critical aspect of successful surgery and has been a matter of concern for over 20 years. Owing to their anti-inflammatory effects and regenerative property, adipose-derived mesenchymal stem cells (AD-MSCs) have been adapted for clinical application in fat grafting, although the mechanism underlying their action remains unclear. Recently, exosomes derived from MSCs were suggested as a better alternative, and these exosomes have also been applied in diverse clinical therapies. Accumulating evidence suggests that MSCs modulate macrophage differentiation via exosome secretion, and the connection between macrophage regulation and the rate of fat graft survival has been established. Here, we identified that let-7c, the key factor in the regulatory process, is shuttled by AD-MSC-derived exosomes to downregulate the transcription factor CCAAT/enhancer-binding protein (C/EBP)-δ. The downregulation of C/EBP-δ resulted in the attenuation of pro-inflammatory M1 macrophages and elevation of anti-inflammatory M2 macrophages. These results suggest that AD-MSC-derived exosomes promote the survival of fat grafts by regulating macrophage polarization via let-7c. This is the first study to elucidate the mechanism underlying the promotion of the fat graft survival rate by AD-MSCs and to evaluate the immunotherapeutic potential of AD-MSC-derived exosomes in fat grafting.</abstract><cop>UK</cop><pub>Oxford University Press</pub><pmid>33704368</pmid><doi>10.1093/abbs/gmab018</doi><tpages>10</tpages></addata></record> |
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title | Exosomes from adipose-derived mesenchymal stem cells promote survival of fat grafts by regulating macrophage polarization via let-7c |
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