Long non‐coding RNA ADAMTS9‐AS1 inhibits the progression of prostate cancer by modulating the miR‐142‐5p/CCND1 axis

Background Emerging evidence has implied the importance of long non‐coding RNAs in cancer development, including prostate cancer (PCa). Bioinformatic analyses have identified that ADAMTS9‐AS1 may play a role in cancer progression. Methods A quantitative real‐time polymerase chain reaction was conduc...

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Bibliographic Details
Published in:The journal of gene medicine 2021-05, Vol.23 (5), p.e3331-n/a
Main Authors: Zhou, Zhien, Wu, Xingcheng, Zhou, Yi, Yan, Weigang
Format: Article
Language:English
Subjects:
ADAMTS9‐AS1
Apoptosis
Apoptosis - genetics
CCND1
Cell growth
Cell Line, Tumor
Cell Movement - genetics
Cell proliferation
Cell Proliferation - genetics
Cyclin D1
Cyclin D1 - genetics
Disease Progression
Gene expression
Gene Expression Regulation, Neoplastic
Gene therapy
Humans
long non‐coding RNA
Male
MicroRNAs - genetics
miRNA
miR‐142‐5p
Non-coding RNA
Polymerase chain reaction
Prostate cancer
Prostatic Neoplasms - genetics
Prostatic Neoplasms - pathology
RNA, Long Noncoding - genetics
Signal Transduction - genetics
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Summary:Background Emerging evidence has implied the importance of long non‐coding RNAs in cancer development, including prostate cancer (PCa). Bioinformatic analyses have identified that ADAMTS9‐AS1 may play a role in cancer progression. Methods A quantitative real‐time polymerase chain reaction was conducted to measure ADAMTS9‐AS1 expression level at messenger RNA level. In vitro functional analyses were performed to investigate cell behaviors status under different conditions. Moreover, rescue assays were conducted to explore the potential mechanisms of ADAMTS9‐AS1 in PCa progression. Results ADAMTS9‐AS1 expression is down‐regulated in PCa. Forcing ADAMTS9‐AS1 expression impedes PCa cell proliferation via initiating cell apoptosis. Importantly, microRNA‐142‐5p (miR‐142‐5p) mimic and small‐interfering RNA targeting cyclin D1 (CCND1, si‐CCND1) could attenuate the inhibitory effects of ADAMTS9‐AS1 overexpression on PCa cell growth. Conclusions Collectively, our results indicate that ADAMTS9‐AS1 suppresses PCa progression by regulating the miR‐142‐5p/CCND1 axis, which provides a new mechanism for the progression of PCa. ADAMTS9‐AS1 serves as a tumor suppressor in prostate cancer by regulating the miR‐142‐5p/CCND1 axis.
ISSN:1099-498X
1521-2254
DOI:10.1002/jgm.3331