Combining NMR-Based Metabolic Profiling and Genome Mining for the Accelerated Discovery of Archangiumide, an Allenic Macrolide from the Myxobacterium Archangium violaceum SDU8

An unprecedented 19-membered allenic macrolide archangiumide (1) was discovered from the myxobacterium Archangium violaceum SDU8 by integrating NMR-based metabolic profiling and genome mining. Its biosynthesis pathway was proposed based on the architectural analysis of the encoding trans-AT PKS gene...

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Bibliographic Details
Published in:Organic letters 2021-03, Vol.23 (6), p.2114-2119
Main Authors: Hu, Jia-Qi, Wang, Jing-Jing, Li, Yue-Lan, Zhuo, Li, Zhang, Ai, Sui, Hai-Yan, Li, Xiao-Ju, Shen, Tao, Yin, Yizhen, Wu, Zhi-Hong, Hu, Wei, Li, Yue-Zhong, Wu, Changsheng
Format: Article
Language:English
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Summary:An unprecedented 19-membered allenic macrolide archangiumide (1) was discovered from the myxobacterium Archangium violaceum SDU8 by integrating NMR-based metabolic profiling and genome mining. Its biosynthesis pathway was proposed based on the architectural analysis of the encoding trans-AT PKS genes and validated by isotope labeling. The methodology of combing 2D NMR-based metabolic profiling and bioinformatics-aided structure prediction, as exemplified by this study, is anticipated to improve discovery efficiency of a broader range of microbial “dark matter”.
ISSN:1523-7060
1523-7052
DOI:10.1021/acs.orglett.1c00265