Study on hemostatic effect and mechanism of starch-based nano-microporous particles

• Published in: International journal of biological macromolecules 2021-05, Vol.179, p.507-518
• Main Authors: Zheng, Caiyun, Bai, Que, Wu, Wendong, Han, Kai, Zeng, Qingyan, Dong, Kai, Zhang, Yanni, Lu, Tingli
• Format: Article
• Language: English
• Subjects: Hemostatic effect; Hemostatic mechanism; Nanoscale porous bioactive glass; Nanoscale porous silica; Starch-based nano-microporous particles
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2021.03.037

The powdered hemostatic particles have broad application prospects in large open wounds, internal organ injuries and penetrating injuries of the body. In this study, nanoscale mescoporous and macroporous silica (MMSN), nanoscale mescoporous and macroporous bioactive glass (MBG), micron-scale cross-linked corn starch porous microspheres (CMS), MMSN@CMS and MBG@CMS starch-based nano-microporous particles were synthesized and their hemostatic effect and hemostatic mechanism were studied. The results showed that comparted with the single particle of CMS, the combination particles MBG@CMS and MMSN@CMS significantly increased the water absorption rate, activated both internal and external coagulation pathways, significantly shortened CBT, as well as the improved hemostatic effects in vitro. The immediately released Ca2+ from MBG@CMS in the blood to participate in the coagulation pathway, and MMSN@CMS activated platelets by concentrating blood coagulation factors, might be the main hemostatic mechanisms for the starch-based nano-microporous particles. Furthermore, the hemostatic efficacy of particles, both in the model of tail-amputation and liver injury in SD rats, showed the starch-based nano-microporous particles, especial MBG@CMS, could significantly reduce the weight of blood loss and shorten the bleeding time. Our research work stated that the starch-based nano-microporous particles MBG@CMS might be a hemostasis biomaterial with the potential applications for the emergency bleeding. •Two kinds of starch-based nano-microporous particles were prepared successfully.•Organic microporous starch ith inorganic nanoparticles was beneficial to synergistic multiple coagulation mechanisms.•Composite particles could trigger both internal and external coagulation pathway.•Starch-based nano-microporous particles could be used for the treatment of bleeding in the epidermis and internal organs.