Single-Atom Ruthenium Biomimetic Enzyme for Simultaneous Electrochemical Detection of Dopamine and Uric Acid

Single-atom catalysts have attracted numerous attention due to the high utilization of metallic atoms, abundant active sites, and highly catalytic activities. Herein, a single-atom ruthenium biomimetic enzyme (Ru-Ala-C3N4) is prepared by dispersing Ru atoms on a carbon nitride support for the simult...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2021-03, Vol.93 (11), p.4916-4923
Main Authors: Xie, Xiaoli, Wang, Dong Ping, Guo, Chunxian, Liu, Yuhang, Rao, Qianghai, Lou, Fangming, Li, Qiannan, Dong, Yongqiang, Li, Qunfang, Yang, Hong Bin, Hu, Fang Xin
Format: Article
Language:English
Subjects:
Biomimetics
Biosensors
Calibration
Carbon nitride
Catalysts
Chemical bonds
Chemistry
Detection limits
Dispersion
Dopamine
Electrochemical analysis
Electrochemistry
Electrons
Enzymes
Morphology
Oxidation
Photoelectron spectroscopy
Photoelectrons
Ruthenium
Scanning transmission electron microscopy
Selectivity
Single atom catalysts
Transmission electron microscopy
Uric acid
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Summary:Single-atom catalysts have attracted numerous attention due to the high utilization of metallic atoms, abundant active sites, and highly catalytic activities. Herein, a single-atom ruthenium biomimetic enzyme (Ru-Ala-C3N4) is prepared by dispersing Ru atoms on a carbon nitride support for the simultaneous electrochemical detection of dopamine (DA) and uric acid (UA), which are coexisting important biological molecules involving in many physiological and pathological aspects. The morphology and elemental states of the single-atom Ru catalyst are studied by transmission electron microscopy, energy dispersive X-ray elemental mapping, high-angle annular dark field–scanning transmission electron microscopy, and high-resolution X-ray photoelectron spectroscopy. Results show that Ru atoms atomically disperse throughout the C3N4 support by Ru–N chemical bonds. The electrochemical characterizations indicate that the Ru-Ala-C3N4 biosensor can simultaneously detect the oxidation of DA and UA with a separation of peak potential of 180 mV with high sensitivity and excellent selectivity. The calibration curves for DA and UA range from 0.06 to 490 and 0.5 to 2135 μM with detection limits of 20 and 170 nM, respectively. Moreover, the biosensor was applied to detect DA and UA in real biological serum samples using the standard addition method with satisfactory results.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.0c05191