Treatment of diabetic mice by microfluidic system-assisted transplantation of stem cells-derived insulin-producing cells transduced with miRNA

Cell-based therapy is a promising approach for the treatment of type-1 diabetes mellitus. Identifying stem cells with differentiation potential to Insulin-producing cells (IPCs) and their application is an emerging issue. Different strategies have been used to support cell survival and their specifi...

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Bibliographic Details
Published in:Life sciences (1973) 2021-06, Vol.274, p.119338-119338, Article 119338
Main Authors: Soltani, Adele, Soleimani, Masoud, Ghiass, Mohammad Adel, Enderami, Seyed Ehsan, Rabbani, Shahram, Jafarian, Arefeh, Allameh, Abdolamir
Format: Article
Language:English
Subjects:
Alginates
Alginic acid
Animals
Anti-miR-7
Blood
Blood glucose
Cell Differentiation
Cell survival
Cell therapy
Collagen
Cytology
Diabetes
Diabetes mellitus
Diabetes Mellitus, Experimental - genetics
Diabetes Mellitus, Experimental - pathology
Diabetes Mellitus, Experimental - therapy
Encapsulation
Glucose
Grafting
Hyperglycemia
Insulin
Insulin - metabolism
Insulin-Secreting Cells - cytology
Insulin-Secreting Cells - metabolism
IPCs
Male
Mice
Mice, Inbred BALB C
Microfluidics
Microfluidics - methods
MicroRNAs - genetics
miR-375
miRNA
Morphology
Stem cell transplantation
Stem Cell Transplantation - methods
Stem cells
Stem Cells - cytology
Survival
Transplantation
Transplants & implants
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Summary:Cell-based therapy is a promising approach for the treatment of type-1 diabetes mellitus. Identifying stem cells with differentiation potential to Insulin-producing cells (IPCs) and their application is an emerging issue. Different strategies have been used to support cell survival and their specific functions to control hyperglycemia conditions. Novel technologies using appropriate materials/fibers can improve cell transplantation. In the present study, IPCs were differentiated from adipose-derived stem cells transduced with miR-375 and anti-miR-7. The cells' survival rate was also improved using a microfluidic system before their in vivo transplantation. After adopting a stable, functional condition of the IPCs, the cells were used for in vivo grafting to diabetic mice, which resulted in a substantial drop in blood glucose during four weeks of grafting compared to the control group (p 
ISSN:0024-3205
1879-0631
DOI:10.1016/j.lfs.2021.119338