Measurement uncertainty in quantifying delta-9-tetrahydrocannabinol (THC) in blood using SPE and LC/MS/MS

•A method for quantifying THC in whole blood using SPE and LC/MS/MS was developed.•Uncertainty components were identified and quantified using a bottom-up approach.•Easy to replicate, step-by-step calculations have been shown.•The Measurement Uncertainty Calculator (MUCalc) is a freely accessible op...

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Bibliographic Details
Published in:Forensic science international 2021-05, Vol.322, p.110744-110744, Article 110744
Main Authors: Klu, Joyce K., Officer, Jane A., Park, Alexandra, Mudie, Roy, NicDaeid, Niamh
Format: Article
Language:English
Subjects:
Blood
Bottom-up approach
Calibration
Calibration curve
Cannabis
Confidence intervals
Fluorides
Forensic sciences
Laboratories
Mathematical analysis
Measurement uncertainty
Method validation
Quality control
Quality standards
Quantitative analysis
Regression analysis
Software
Solid phases
Tetrahydrocannabinol
THC
THC, LC/MS/MS
Uncertainty
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Summary:•A method for quantifying THC in whole blood using SPE and LC/MS/MS was developed.•Uncertainty components were identified and quantified using a bottom-up approach.•Easy to replicate, step-by-step calculations have been shown.•The Measurement Uncertainty Calculator (MUCalc) is a freely accessible open source software. A method for the quantitative analysis of delta-9-tetrahydrocannabinol (THC, the main active ingredient of cannabis) in whole blood using solid phase extraction and LC/MS/MS has been developed. A bottom-up approach with method validation data was used to evaluate and estimate the measurement uncertainty (MU) of the analytical method. The sources of uncertainty were identified using a cause and effect diagram. The contribution of each uncertainty component was estimated and were combined to derive the overall uncertainty of the analytical method. The combined uncertainty was estimated to be 0.131 μg/L (
ISSN:0379-0738
1872-6283
DOI:10.1016/j.forsciint.2021.110744