Soluble inflammatory mediators of synoviocytes stimulated by monosodium urate crystals induce the production of oxidative stress, pain, and inflammation mediators in chondrocytes: Secretome of synoviocytes induces chondrocyte damage

• Published in: Clinical rheumatology 2021-08, Vol.40 (8), p.3265-3271
• Main Authors: López-Reyes, Alberto, Medina-Luna, Daniel, Santamaría-Olmedo, Mónica, Martínez-Flores, Karina, Zamudio-Cuevas, Yessica, Fernández-Torres, Javier, Martínez-Nava, Gabriela Angélica, Olivos-Meza, Anell, Camacho-Rea, Carmen, Fernández-Moreno, Mercedes, Blanco, Francisco J., Pineda, Carlos
• Format: Article
• Language: English
• Subjects: Brief Report; Medicine; Medicine & Public Health; Rheumatology
• ISSN: 0770-3198; 1434-9949
• DOI: 10.1007/s10067-021-05676-w

We hypothesized that the secretion of inflammatory mediators from synoviocytes affects the chondrocyte homeostasis of articular cartilage. This study was a preliminary attempt to elucidate the molecular mechanisms by which soluble mediators obtained from activated synoviocytes induce oxidative stress and inflammation in chondrocytes. We measured the concentrations of interleukin-6 (IL-6), interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1), nerve growth factor (NGF), superoxide anion (O 2 •− ), hydrogen peroxide (H 2 O 2 ), and nitric oxide (NO • ) from articular human cells. First, we created a conditional basal medium by exposing synoviocytes (HS) to monosodium urate crystals (CBM). The chondrocytes were exposed to either CBM (CCM), urate crystals directly (CMSU), or remained untreated (CC) as a negative control. Data were analyzed by ANOVA tests; Bonferroni test was performed for multiple comparisons between groups. Interestingly, we observed that mediators of inflammation and oxidative stress were significantly higher in CCM than CMSU and CC groups ( P