ELF1 Transcription Factor Enhances the Progression of Glioma via ATF5 promoter

A key transcriptional activator, activating transcription factor 5 (ATF5), is aberrantly overexpressed in glioma and supports both poor prognosis and antiapototic potential. Unfortunately, data on ATF5 is largely based on its regulatory mechanism. Further investigation of the upstream regulatory fac...

Full description

Saved in:
Bibliographic Details
Published in:ACS chemical neuroscience 2021-04, Vol.12 (7), p.1252-1261
Main Authors: Hu, Ming, Li, Huanting, Xie, Hongwei, Fan, Mingchao, Wang, Jianpeng, Zhang, Niankai, Ma, Junwei, Che, Shusheng
Format: Article
Language:English
Subjects:
Activating Transcription Factors - genetics
Activating Transcription Factors - metabolism
Cell Line, Tumor
Cyclic AMP Response Element-Binding Protein
Glioma - genetics
Humans
Nuclear Proteins
Promoter Regions, Genetic
Transcription Factors - genetics
Up-Regulation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A key transcriptional activator, activating transcription factor 5 (ATF5), is aberrantly overexpressed in glioma and supports both poor prognosis and antiapototic potential. Unfortunately, data on ATF5 is largely based on its regulatory mechanism. Further investigation of the upstream regulatory factor for ATF5 transcription in glioma is required. Clinical data for patients with diagnosed glioma were obtained from The Cancer Genome Atlas (TCGA). Additionally, transcription factors potentially regulating the ATF5 promoter in glioma were screened with bioinformatics. A further experimental study was performed to investigate both the role of E74-like factor 1 (ELF1) and the binding of ELF1 and the ATF5 promoter in glioma. We show that ATF5 expression is upregulated in glioma tissues and associated with tumor malignancy and worse prognosis. As a putative upstream regulator, silencing ELF1 inhibits glioma cell growth and migration with ATF5 involvement. Moreover, ELF1 upregulation is also associated with poor prognosis in glioma. Importantly, the luciferase assay and chromatin immunoprecipitation (ChIP) reveal that the ATF5 gene promoter is essential for ELF1-dependent activation of ATF5 gene transcription. These results indicate that a high expression of ELF1 may be related to the malignant behavior of human glioma and ELF1 promotes glioma development mediated by transactivation of the ATF5 gene.
ISSN:1948-7193
1948-7193
DOI:10.1021/acschemneuro.1c00070